Coppari Sofia, Colomba Mariastella, Fraternale Daniele, Brinkmann Vanessa, Romeo Margherita, Rocchi Marco Bruno Luigi, Di Giacomo Barbara, Mari Michele, Guidi Loretta, Ramakrishna Seeram, Ventura Natascia, Albertini Maria Cristina
Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, Italy.
Medical Faculty, Institute of Clinical Chemistry and Laboratory Diagnostic, Heinrich Heine University and the IUF- Leibniz Research Institute for Environmental Medicine Auf'm Hennekamp 50, 40225 Düsseldorf, Germany.
Antioxidants (Basel). 2021 Mar 2;10(3):374. doi: 10.3390/antiox10030374.
fruit (PSF) ethanol extract, showing a peculiar content of biologically active molecules (polyphenols), was investigated for its wound healing capacity, a typical feature that declines during aging and is negatively affected by the persistence of inflammation and oxidative stress. To this aim, first, PSF anti-inflammatory properties were tested on young and senescent LPS-treated human umbilical vein endothelial cells (HUVECs). As a result, PSF treatment increased miR-146a and decreased IRAK-1 and IL-6 expression levels. In addition, the PSF antioxidant effect was validated in vitro with DPPH assay and confirmed by in vivo treatments in . Our findings showed beneficial effects on worms' lifespan and healthspan with positive outcomes on longevity markers (i.e., miR-124 upregulation and miR-39 downregulation) as well. The PSF effect on wound healing was tested using the same cells and experimental conditions employed to investigate PSF antioxidant and anti-inflammaging ability. PSF treatment resulted in a significant improvement of wound healing closure (ca. 70%), through cell migration, both in young and older cells, associated to a downregulation of inflammation markers. In conclusion, PSF extract antioxidant and anti-inflammaging abilities result in improved wound healing capacity, thus suggesting that PSF might be helpful to improve the quality of life for its beneficial health effects.
水果（PSF）乙醇提取物含有独特的生物活性分子（多酚），对其伤口愈合能力进行了研究。伤口愈合是一种典型特征，在衰老过程中会下降，并且会受到炎症和氧化应激持续存在的负面影响。为此，首先在年轻和衰老的经脂多糖处理的人脐静脉内皮细胞（HUVECs）上测试了PSF的抗炎特性。结果，PSF处理增加了miR-146a的表达，降低了IRAK-1和IL-6的表达水平。此外，通过DPPH试验在体外验证了PSF的抗氧化作用，并在体内处理中得到证实。我们的研究结果表明，PSF对蠕虫的寿命和健康寿命有有益影响，对长寿标志物（即miR-124上调和miR-39下调）也有积极影响。使用与研究PSF抗氧化和抗炎症衰老能力相同的细胞和实验条件测试了PSF对伤口愈合的影响。PSF处理通过细胞迁移使年轻和老年细胞的伤口愈合闭合率显著提高（约70%），这与炎症标志物的下调有关。总之，PSF提取物的抗氧化和抗炎症衰老能力导致伤口愈合能力提高，因此表明PSF因其有益的健康影响可能有助于改善生活质量。
