Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece.
J Appl Microbiol. 2010 Dec;109(6):1929-36. doi: 10.1111/j.1365-2672.2010.04822.x.
The purpose of this study was to investigate the role of H(2) O(2) and the related oxidative stress markers catalase (CAT) and lipid peroxidation in the sclerotial differentiation of the phytopathogenic filamentous fungi Sclerotium rolfsii, Sclerotinia minor, Sclerotinia sclerotiorum and Rhizoctonia solani.
Using the H(2) O(2) -specific scopoletin fluorometric assay and the CAT-dependent H(2) O(2) consumption assays, it was found that the production rate of intra/extracellular H(2) O(2) and CAT levels in the sclerotiogenic fungi were significantly higher and lower, respectively, than those of their nondifferentiating counterpart strains. They peaked in the transition between the undifferentiated and the differentiated state of the sclerotiogenic strains, suggesting both a cell proliferative and differentiative role. In addition, the indirect indicator of oxidative stress, lipid peroxidation, was substantially decreased in the nondifferentiating strains.
These findings suggest that the differentiative role of H(2) O(2) is expressed via induction of higher oxidative stress in the sclerotiogenic filamentous phytopathogenic fungi.
This study shows that the direct marker of oxidative stress H(2) O(2) is involved in the sclerotial differentiation of the phytopathogenic filamentous fungi S. rolfsii, S. minor, S. sclerotiorum and R. solani, which could have potential biotechnological implications in terms of developing antifungal strategies by regulating intracellular H(2) O(2) levels.
本研究旨在探讨 H(2)O(2) 及其相关氧化应激标志物过氧化氢酶 (CAT) 和脂质过氧化作用在植物病原丝状真菌罗耳伏革菌、小核盘菌、核盘菌和立枯丝核菌的菌核分化中的作用。
使用 H(2)O(2)特异性荧光素酶比色法和依赖 CAT 的 H(2)O(2)消耗测定法，发现产生活性氧（H(2)O(2)）的速率和 CAT 水平在菌核形成真菌中的胞内外水平明显更高和更低，分别与它们的非分化对照菌株相比。它们在菌核形成真菌的未分化和分化状态之间的过渡阶段达到峰值，表明两者都具有细胞增殖和分化作用。此外，非分化菌株中的氧化应激间接指标脂质过氧化作用显著降低。
这些发现表明，H(2)O(2) 的分化作用是通过诱导菌核形成丝状植物病原真菌中更高的氧化应激来表达的。
本研究表明，氧化应激的直接标志物 H(2)O(2) 参与了植物病原丝状真菌罗耳伏革菌、小核盘菌、核盘菌和立枯丝核菌的菌核分化，这可能在通过调节细胞内 H(2)O(2) 水平来开发抗真菌策略方面具有潜在的生物技术意义。
