无细胞循环 DNA：在肾细胞癌患者中的诊断价值。

Cell-free circulating DNA: Diagnostic value in patients with renal cell cancer.

机构信息

Department of Urology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.

出版信息

Anticancer Res. 2010 Jul;30(7):2785-9.

PMID:20683013

Abstract

OBJECTIVES

To analyse the diagnostic and prognostic value of cell-free DNA in patients with renal cell carcinoma (RCC).

PATIENTS AND METHODS

Cell-free DNA was measured in 35 patients with RCC and 54 healthy individuals using quantitative real-time PCR. ACTB-106 detects fragmented cell-free DNA due to apoptosis and ACTB-384 detects long DNA fragments by necrosis. DNA-Integrity (ACTB-384/ACTB-106 ratio) served as measure of DNA fragmentation.

RESULTS

Levels of both DNA fragments were increased in RCC patients compared to healthy individuals (ACTB-384: 1.77 vs. 0.61ng/ml, p=0.0003; ACTB-106: 1.31ng/ml vs. 0.77 ng/ml p=0.003). Receiver operator characteristic analysis (ROC) showed at a threshold level of 1.03 ng/ml for ACTB-106 68.6%, sensitivity and 70.4% specificity (AUC: 0.69). ROC analysis showed at a threshold level of 1.70 ng/ml for ACTB-384 57.1%, sensitivity and 81.5% specificity (AUC: 0.73). DNA integrity was increased in RCC (1.07 vs. 0.72 p=0.04). In vascular invasion the DNA integrity was reduced (p=0.003).

CONCLUSION

Cell-free-DNA levels are increased in RCC. The DNA integrity indicates mostly necrotic origin in RCC.

摘要

目的

分析游离 DNA 在肾细胞癌 (RCC) 患者中的诊断和预后价值。

患者和方法

使用实时定量 PCR 法检测 35 例 RCC 患者和 54 例健康个体的游离 DNA。ACTB-106 检测凋亡导致的游离 DNA 片段化，ACTB-384 检测坏死引起的长 DNA 片段。DNA 完整性（ACTB-384/ACTB-106 比值）作为 DNA 片段化的衡量指标。

结果

与健康个体相比，RCC 患者的两种 DNA 片段水平均升高（ACTB-384：1.77 比 0.61ng/ml，p=0.0003；ACTB-106：1.31ng/ml 比 0.77ng/ml，p=0.003）。接受者操作特征分析（ROC）显示，ACTB-106 的截断值为 1.03ng/ml 时，敏感性为 68.6%，特异性为 70.4%（AUC：0.69）。ROC 分析显示，ACTB-384 的截断值为 1.70ng/ml 时，敏感性为 57.1%，特异性为 81.5%（AUC：0.73）。RCC 中 DNA 完整性增加（1.07 比 0.72，p=0.04）。血管侵犯时 DNA 完整性降低（p=0.003）。