胆固醇硫酸酯稳定 DMPC/DHPC 双胶束
Thermal stabilization of DMPC/DHPC bicelles by addition of cholesterol sulfate.
机构信息
Department of Physics, University of California, Irvine, California 92697, USA.
出版信息
J Am Chem Soc. 2010 Aug 25;132(33):11406-7. doi: 10.1021/ja1041012.
Abstract
Doping DMPC/DHPC bicelles with cholesterol sulfate broadens the temperature range over which stable alignment occurs, forming an aligned phase at lower temperatures even with high lipid concentrations. Cholesterol sulfate appears to combine the advantages of cholesterol with those of charged amphiphiles, stabilizing the aligned phase and preventing precipitation. This allows NMR data for RDC and CSA protein structure constraints to be acquired at or below room temperature, an obvious advantage for solid-state and solution studies of heat-sensitive proteins.
摘要
用硫酸胆固醇掺杂 DMPC/DHPC 双分子层可以拓宽稳定排列发生的温度范围，即使在高脂质浓度下，也能在较低温度下形成排列相。硫酸胆固醇似乎结合了胆固醇和带电两亲物的优点，稳定了排列相并防止沉淀。这使得可以在室温或以下获得 RDC 和 CSA 蛋白质结构约束的 NMR 数据，这对于热敏感蛋白质的固态和溶液研究是一个明显的优势。
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