Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221-0172, USA.
Anal Chem. 2010 Sep 1;82(17):7430-5. doi: 10.1021/ac101579m.
We have developed a simple, rapid, and sensitive carbohydrate-based magnetic relaxation switch assay for the detection of carbohydrate binding proteins. This technique was used to detect lectins and toxins that are known to bind to specific carbohydrates. Lectins that bind to the same carbohydrate displayed differential aggregation profiles because of differences in the structure and number of binding sites of the lectins. We demonstrated that selectivity and sensitivity can be enhanced using two different recognition elements. We have also demonstrated that magnetic relaxation switch assays can be used to detect toxins in a complex medium such as stool and environmental samples.
我们开发了一种简单、快速且灵敏的基于碳水化合物的磁共振弛豫开关分析方法，用于检测碳水化合物结合蛋白。该技术用于检测已知与特定碳水化合物结合的凝集素和毒素。由于凝集素的结构和结合位点数量的不同，与同一碳水化合物结合的凝集素显示出不同的聚集谱。我们证明了使用两种不同的识别元件可以提高选择性和灵敏度。我们还证明了磁共振弛豫开关分析可以用于检测复杂介质（如粪便和环境样本）中的毒素。
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