磁弛豫切换传感的机理。
Mechanism of magnetic relaxation switching sensing.
机构信息
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
出版信息
ACS Nano. 2012 Aug 28;6(8):6821-8. doi: 10.1021/nn301615b. Epub 2012 Jul 10.
Abstract
Magnetic relaxation switching (MRSw) assays that employ target-induced aggregation (or disaggregation) of magnetic nanoparticles (MNPs) can be used to detect a wide range of biomolecules. The precise working mechanisms, however, remain poorly understood, often leading to confounding interpretation. We herein present a systematic and comprehensive characterization of MRSw sensing. By using different types of MNPs with varying physical properties, we analyzed the nature and transverse relaxation modes for MRSw detection. The study found that clustered MNPs are universally in a diffusion-limited fractal state (dimension of ~2.4). Importantly, a new model for transverse relaxation was constructed that accurately recapitulates observed MRSw phenomena and predicts the MRSw detection sensitivities and dynamic ranges.
摘要
基于磁纳米粒子（MNPs）目标诱导聚集（或解聚集）的磁弛豫切换（MRSw）检测可用于检测多种生物分子。然而，其确切的工作机制仍了解甚少，这往往导致结果解释混乱。本研究对 MRSw 检测进行了系统全面的特征分析。通过使用具有不同物理性质的不同类型的 MNPs，我们分析了 MRSw 检测的性质和横向弛豫模式。研究发现，聚集的 MNPs 普遍处于扩散受限的分形状态（维度约为 2.4）。重要的是，构建了一个新的横向弛豫模型，该模型准确地再现了观察到的 MRSw 现象，并预测了 MRSw 检测的灵敏度和动态范围。
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