Department of Medical Genetics and Fetal Medicine, Palacký University Medical School, Olomouc, Czech Republic.
Int J Gynecol Cancer. 2010 May;20(4):576-82. doi: 10.1111/IGC.0b013e3181d80ac4.
The objectives of this study were to assess the immunohistochemical expression of p53, bcl-2, c-erbB-2, Ki-67, estrogen (ER) and progesterone (PR) receptors, matrix metalloproteinase-7 and -26 (MMP-7 and MMP-26) in endometrial cancer patients and to assess the relation between steroid receptor positivity and other markers.
Experimental prospective study.
Department of Obstetrics and Gynecology, Department of Genetics, Department of Pathology, Palacký University Medical School and University Hospital Olomouc.
We studied 144 cases of primary untreated endometrial carcinoma in which the p53, bcl-2, c-erbB-2, Ki-67, ER, PR, MMP-7, and MMP-26 antigens were investigated with the use of immunohistochemical methods. We evaluated the correlations among immunohistochemical staining and the age, International Federation of Gynecology and Obstetrics stage, grading, depth of invasion, and metastatic spread to lymph nodes.
Mean age was 65.7 years (range, 34-90 years). p53, bcl-2, c-erbB-2, Ki-67, ER, and PR were positive in 35 (24.3%), 100 (69.4%), 41 (28.4%), 65 (45.1%), 115 (79.8%), and 127 (88.1%) cases, respectively. Matrix metalloproteinases were evaluated in a group of 70 patients, wherein MMP-7 was positive in 33 patients (47.1%) and MMP-26 was positive in 40 patients (57.1%). The expression of MMP-7 decreased with higher patient age. p53 and Ki-67 overexpression was found to be related to poor differentiation. Immunostaining for bcl-2 correlated with the positivity of steroid receptors status, whereas immunostaining for c-erbB-2 correlated inversely with ER-positive group of cases.
The overexpression of p53 and Ki-67 seems to indicate a more malignant phenotype, whereas bcl-2 expression in dependence of steroid receptor positivity could contribute to the identification of high-risk tumors.
本研究旨在评估子宫内膜癌患者中 p53、bcl-2、c-erbB-2、Ki-67、雌激素（ER）和孕激素（PR）受体、基质金属蛋白酶-7 和 -26（MMP-7 和 MMP-26）的免疫组织化学表达，并评估甾体受体阳性与其他标志物之间的关系。
实验性前瞻性研究。
帕尔杜比采大学医学院和奥洛穆茨大学医院妇产科、遗传学系和病理学系。
我们研究了 144 例未经治疗的原发性子宫内膜癌患者，使用免疫组织化学方法检测 p53、bcl-2、c-erbB-2、Ki-67、ER、PR、MMP-7 和 MMP-26 抗原。我们评估了免疫组化染色与年龄、国际妇产科联合会（FIGO）分期、分级、浸润深度和淋巴结转移之间的相关性。
平均年龄为 65.7 岁（范围 34-90 岁）。p53、bcl-2、c-erbB-2、Ki-67、ER 和 PR 阳性的患者分别为 35 例（24.3%）、100 例（69.4%）、41 例（28.4%）、65 例（45.1%）、115 例（79.8%）和 127 例（88.1%）。我们在一组 70 例患者中评估了基质金属蛋白酶，其中 MMP-7 阳性的患者有 33 例（47.1%），MMP-26 阳性的患者有 40 例（57.1%）。MMP-7 的表达随着患者年龄的增加而降低。p53 和 Ki-67 的过度表达与分化不良有关。bcl-2 的免疫组化染色与甾体受体阳性状态相关，而 c-erbB-2 的免疫组化染色与 ER 阳性组呈负相关。
p53 和 Ki-67 的过度表达似乎表明更具恶性表型，而 bcl-2 的表达依赖于甾体受体阳性可能有助于识别高危肿瘤。
