Howland Linda J, Haynes Nicole M, Darcy Phillip K
Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Australia.
Methods Mol Biol. 2010;651:291-306. doi: 10.1007/978-1-60761-786-0_17.
Gene modification of T cells with chimeric T-cell receptor (TCR) transgenes offers a novel way to generate tumor-specific T cells for cancer immunotherapy. Retroviruses have been utilized as the most common means of efficiently transducing primary T lymphocytes with these transgenes. In this section we describe methods for generation of chimeric TCR's and utilization of retroviral vectors for efficient transduction of these transgenes in primary mouse T lymphocytes.
用嵌合型T细胞受体（TCR）转基因对T细胞进行基因改造，为癌症免疫治疗产生肿瘤特异性T细胞提供了一种新方法。逆转录病毒已被用作将这些转基因有效转导至原代T淋巴细胞的最常用手段。在本节中，我们描述嵌合型TCR的生成方法以及利用逆转录病毒载体在原代小鼠T淋巴细胞中有效转导这些转基因的方法。
