Pediatric Gastroenterology Department, Federal University of Sao Paulo (UNIFESP), Rua Pedro de Toledo, 441, CEP 04039-031 Sao Paulo-SP, Brazil.
Br J Nutr. 2010 Nov;104(10):1471-6. doi: 10.1017/S0007114510002497. Epub 2010 Aug 6.
There is concern regarding the possible negative effects of ingestion of dietary fibre on growth and intestinal Fe absorption in infants. The aim of the present study was to compare the effect of a fibre mixture on the growth and the intestinal absorption of Fe in rats with Fe-deficiency anaemia with that of a diet without fibres. Faecal weight and caecal pH were also evaluated. According to the Hb depletion-repletion model, twenty-two male weaned Wistar rats were fed the AIN93-G diet without Fe until Fe-deficiency anaemia was induced with Hb < 70 g/l. The anaemic rats were divided into two groups: (1) fibre mixture group--fed 100 g of fibre mixture/kg of diet (soya polysaccharide, inulin, resistant starch, Arabic gum, fructo-oligossaccharide and cellulose) (n 11); (2) control group--fed without fibres (n 11). All diets had 157 mg of ferric citrate (30 mg of elemental Fe) added to lead to recovery from anaemia. Fe intestinal absorption was measured by Hb repletion efficiency (HRE) and apparent Fe intestinal absorption. The HRE was 44.8 (SD 9.5) % in the fibre mixture group and 43.0 (SD 9.5) % in the control group (P = 0.664). The apparent Fe absorption was 46.2 (SD 16.5) and 47.2 (SD 10.2) % (P = 0.861) in the fibre mixture and control groups, respectively. The faecal weight median was 6.17 g in the fibre mixture group and 2.11 g in the control group (P < 0.001). The caecal pH was in the same order: 6.11 (SD 0.59) and 7.07 (SD 0.34) (P < 0.001). Both the groups consumed similar quantities of diet, and growth was similar in both the groups. The fibre mixture had no influence either on growth or on Fe intestinal absorption in rats recovering from anaemia. This mixture favoured an increase in faecal weight and a decrease in caecal pH.
人们担心膳食纤维的摄入可能会对婴儿的生长和肠道铁吸收产生负面影响。本研究的目的是比较纤维混合物对缺铁性贫血大鼠的生长和肠道铁吸收的影响，以及不含纤维的饮食的影响。还评估了粪便重量和盲肠 pH 值。根据血红蛋白耗竭-补充模型，22 只雄性断奶 Wistar 大鼠在缺铁性贫血发生前用不含铁的 AIN93-G 饮食喂养，直至血红蛋白 < 70 g/l 导致贫血。将贫血大鼠分为两组：（1）纤维混合物组——喂养 100 g 纤维混合物/公斤饮食（大豆多糖、菊粉、抗性淀粉、阿拉伯胶、低聚果糖和纤维素）（n 11）；（2）对照组——不喂纤维（n 11）。所有饮食均添加 157 mg 柠檬酸铁（30 mg 元素铁）以从贫血中恢复。通过血红蛋白补充效率（HRE）和表观铁肠道吸收率来测量铁肠道吸收。纤维混合物组的 HRE 为 44.8（SD 9.5）%，对照组为 43.0（SD 9.5）%（P = 0.664）。纤维混合物组和对照组的表观铁吸收率分别为 46.2（SD 16.5）%和 47.2（SD 10.2）%（P = 0.861）。纤维混合物组的粪便重量中位数为 6.17 g，对照组为 2.11 g（P < 0.001）。盲肠 pH 值也相同：6.11（SD 0.59）和 7.07（SD 0.34）（P < 0.001）。两组均摄入相似量的饮食，且两组的生长情况相似。纤维混合物对从贫血中恢复的大鼠的生长和肠道铁吸收均无影响。这种混合物有利于粪便重量的增加和盲肠 pH 值的降低。
