暴露于草酸盐和一水合草酸钙晶体的NRK-52E细胞中基质Gla蛋白的表达。
Matrix Gla protein expression in NRK-52E cells exposed to oxalate and calcium oxalate monohydrate crystals.
作者信息
Gao Bing, Yasui Takahiro, Lu Xiuli, Zhou Hongge, Liu Jian, Liu Ping, Okada Atsushi, Xiao Chunling, Kohri Kenjiro
机构信息
Department of Cell Biology and Genetics, Shenyang Medical College, Shenyang, China.
出版信息
Urol Int. 2010;85(2):237-41. doi: 10.1159/000314947. Epub 2010 Aug 4.
PURPOSE
Matrix Gla protein (MGP), a potent calcification inhibitor in extracellular matrix, is a molecular determinant regulating vascular calcification. Here, we characterized MGP expression in cultured renal tubular epithelial cells exposed to oxalate and calcium oxalate monohydrate (COM) crystals.
METHODS
NRK-52E cells were cultured and exposed to medium containing oxalate or COM for 1, 3, 6, 12 and 24 h. The levels of MGP mRNA expression were quantified using real-time PCR (RT-PCR), and the levels of protein expression were characterized by Western blot.
RESULTS
MGP was expressed rapidly and intensively following exposure to COM and was time-dependent with exposure to oxalate.
CONCLUSION
Our results have given a profile of MGP expression in renal tubular epithelial cells exposed to oxalate and crystals and provided support for an association between MGP and kidney stones. Further investigations on the mechanism of the MGP in the process of crystal-cell interaction may be important in the future.
目的
基质Gla蛋白（MGP）是细胞外基质中一种有效的钙化抑制剂，是调节血管钙化的分子决定因素。在此，我们对暴露于草酸盐和一水合草酸钙（COM）晶体的培养肾小管上皮细胞中MGP的表达进行了表征。
方法
培养NRK-52E细胞，并将其暴露于含有草酸盐或COM的培养基中1、3、6、12和24小时。使用实时PCR（RT-PCR）定量MGP mRNA表达水平，并通过蛋白质印迹法表征蛋白质表达水平。
结果
暴露于COM后，MGP迅速且强烈表达，并且与暴露于草酸盐的时间相关。
结论
我们的结果描绘了暴露于草酸盐和晶体的肾小管上皮细胞中MGP的表达情况，并为MGP与肾结石之间的关联提供了支持。未来对MGP在晶体-细胞相互作用过程中机制的进一步研究可能很重要。
Zotero 插件