一种用于检测人血清储备胆红素负荷能力的自旋分析方法的开发。
Development of a spin assay for reserve bilirubin loading capacity of human serum.
作者信息
Hsia J C, Kwan N H, Er S S, Wood D J, Chance G W
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1542-5. doi: 10.1073/pnas.75.3.1542.
The capacity of human serum albumin to bind bilirubin is of paramount importance in the prevention of neurological damage due to hyperbilirubinemia in the neonate. Currently, there is no rapid, reliable method to determine the reserve bilirubin loading capacity of serum albumin. A method using electron spin resonance spectroscopy with a dianionic spin-label molecular probe [1-N-(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl)-5-N-(1-aspartate)-2,4-dinitrobenzene] is presented which yields the reserve binding capacity of the bilirubin high-affinity binding site on human serum albumin and reserve bilirubin loading capacity of serum in mg/dl. Clinical validation of this spin assay may improve the neonatal care of jaundiced newborns.
人血清白蛋白结合胆红素的能力对于预防新生儿高胆红素血症所致的神经损伤至关重要。目前，尚无快速、可靠的方法来测定血清白蛋白的储备胆红素结合能力。本文介绍了一种使用电子自旋共振光谱法和双阴离子自旋标记分子探针[1-N-(2,2,6,6-四甲基-1-氧代-4-哌啶基)-5-N-(1-天冬氨酸)-2,4-二硝基苯]的方法，该方法可得出人血清白蛋白上胆红素高亲和力结合位点的储备结合能力以及血清中以mg/dl为单位的储备胆红素结合能力。这种自旋测定法的临床验证可能会改善黄疸新生儿的护理。
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