Department of Immunology, Xinxiang Medical University, Xingxiang, China.
Cancer Invest. 2010 Dec;28(10):1019-23. doi: 10.3109/07357900902849608. Epub 2010 Aug 6.
To investigate the effects of fragile histidine triad (FHIT) restoration on cell proliferation and apoptosis in human cutaneous T-cell lymphoma cell line, Hut-78, in vitro and in nude mouse. Wild-type FHIT gene was transfected by liposome into the Hut-78 cells. The alteration of cell growth curve and soft agar colony formation was studied in vitro and in nude mice. The FHIT gene was stably expressed in Hut-78 cells after the transfection. Compared with the controls, restoration of FHIT expression inhibited cell growth and induced apoptosis. Tumor formation in vivo was strongly suppressed by FHIT gene restoration. Our data demonstrate restoration of FHIT gene expression inhibit the tumor cell growth and provide an option for the treatment.
为了研究脆性组氨酸三联体（FHIT）的恢复对体外和裸鼠人皮肤 T 细胞淋巴瘤细胞系 Hut-78 细胞增殖和凋亡的影响，用脂质体将野生型 FHIT 基因转染入 Hut-78 细胞。研究了体外细胞生长曲线和软琼脂集落形成的变化以及裸鼠体内。转染后，Hut-78 细胞中 FHIT 基因稳定表达。与对照组相比，FHIT 表达的恢复抑制了细胞生长并诱导了细胞凋亡。FHIT 基因恢复强烈抑制了体内肿瘤的形成。我们的数据表明 FHIT 基因表达的恢复抑制了肿瘤细胞的生长，并为治疗提供了一种选择。
