Department of Aquaculture, Tamilnadu Veterinary & Animal Sciences University, Fisheries College and Research Institute, Tuticorin, India.
J Fish Dis. 2010 Sep;33(9):749-58. doi: 10.1111/j.1365-2761.2010.01181.x.
To detect genomic variation of white spot syndrome virus (WSSV) isolates from different geographical regions of India, the variable number of the tandem repeat (VNTR) region of the ORF 94 (Thailand WSSV isolate - GeneBank Accession No. AF369029) was analysed using five specific sets of primers. Analysis of 70 WSSV-positive samples showed the presence of 14 different genotypes of WSSV with VNTRs ranging from 2 to 16 tandem repeats with the majority (85.47%) having 6-12 tandem repeats. Occurrence of different genotypes of WSSV was found to be neither correlated to any specific geographical region nor to the different growth stage of the tiger shrimp, Penaeus monodon. Pathogenicity studies conducted with 25 isolates of WSSV revealed the presence of virulent and avirulent strains of WSSV in Indian shrimp farms. However, an unambiguous link could not be established between the different genotypes and their virulence.
为了检测来自印度不同地理区域的白斑综合征病毒（WSSV）分离株的基因组变异，使用五组特定的引物分析了 ORF94 的串联重复（VNTR）区域。对 70 个 WSSV 阳性样本的分析显示，存在 14 种不同基因型的 WSSV，VNTR 从 2 到 16 个串联重复，其中大多数（85.47%）有 6-12 个串联重复。WSSV 的不同基因型的出现既与特定的地理区域无关，也与虎虾（Penaeus monodon）的不同生长阶段无关。对 25 个 WSSV 分离株进行的致病性研究表明，在印度虾养殖场存在毒力和无毒力的 WSSV 菌株。然而，不同基因型与其毒力之间的关系尚无法明确。
