Miller M L, Fishleder A J, Tubbs R R
Cleveland Clinic Foundation, Department of Laboratory Hematology, Ohio 44195-5139.
Am J Clin Pathol. 1991 Jul;96(1):100-8. doi: 10.1093/ajcp/96.1.100.
The monoclonal antibodies (MoAbs) CD22 and CD11c recognize B-lymphocyte- and monocyte-associated antigens, respectively. Reports indicate that when these two MoAbs co-express, they represent a unique marker for hairy cell leukemia (HCL) although neither is specific for that disease. The authors evaluated the expression and diagnostic utility of CD22 and CD11C in specimens from 26 normal subjects, 29 patients, with various nonlymphoproliferative disorders (NLPDs), and 75 patients with different types of chronic lymphoproliferative disorders (CLDs) using two-color flow cytometric analysis of peripheral blood lymphocytes. Lymphocytes co-expressed CD22 and CD11c in less than or equal to 3% of the normal subjects and in less than or equal to 6% of the patients with NLPDs. These markers were expressed in greater than 10% of the lymphocytes of 46% (32/69) of the patients with B-cell CLDs: B-cell chronic-lymphocytic leukemia, 9/41; B-cell non-Hodgkin's lymphoma, 8/14; HCL, 11/11; B-cell lymphoproliferative disorder (NOS), 1/2; and B-cell prolymphocytic leukemia, 1/1. None (0/6) of the lymphocytes of patients with T-cell CLDs expressed greater than 10% CD22-positive (CD22+) or CD11c-positive (CD11c+) cells. The HCL cases demonstrated a unique CD22+CD11c+ fluorescence histogram pattern, distinct from other lymphoproliferative disorders, that was characterized by uniformly intense CD11c and CD22 fluorescence. Differences in the expression of the CD22+CD11C- and CD22+CD11C+ phenotypes between diagnostic groups were found, most notable was a paucity of CD22+CD11c+ cells in lymphocytes of patients with HCL. CD22 also had more variable expression than CD19 and HLA-DR in the cases of B-cell CLD. This study demonstrates that the CD22+CD11c+ phenotype is not unique to HCL but is a consistent feature of that disorder and that the immunofluorescence pattern of co-expression in HCL is diagnostically useful.
单克隆抗体（MoAbs）CD22和CD11c分别识别与B淋巴细胞和单核细胞相关的抗原。报告表明，当这两种单克隆抗体共表达时，它们代表毛细胞白血病（HCL）的一种独特标志物，尽管两者都不是该疾病的特异性标志物。作者使用外周血淋巴细胞双色流式细胞术分析，评估了26名正常受试者、29名患有各种非淋巴细胞增殖性疾病（NLPDs）的患者以及75名患有不同类型慢性淋巴细胞增殖性疾病（CLDs）的患者标本中CD22和CD11C的表达及诊断效用。正常受试者中小于或等于3%的淋巴细胞以及NLPDs患者中小于或等于6%的淋巴细胞共表达CD22和CD11c。在46%（32/69）的B细胞CLDs患者的淋巴细胞中，这些标志物的表达超过10%：B细胞慢性淋巴细胞白血病，9/41；B细胞非霍奇金淋巴瘤，8/14；毛细胞白血病，11/11；B细胞淋巴细胞增殖性疾病（未分类），1/2；以及B细胞原淋巴细胞白血病，1/1。T细胞CLDs患者的淋巴细胞中无一例（0/6）表达超过10%的CD22阳性（CD22+）或CD11c阳性（CD11c+）细胞。毛细胞白血病病例表现出一种独特的CD
