Avagyan Serine, Amrani Yacine M, Snoeck Hans-Willem
Department of Gene and Cell Medicine, Mount Sinai of School of Medicine, New York, USA.
Methods Enzymol. 2010;476:429-47. doi: 10.1016/S0076-6879(10)76023-7.
Hematopoietic stem cells (HSCs) can self-renew and give rise to all the cells of the blood and the immune system. As they differentiate, HSCs progressively lose their self-renewal capacity and generate lineage-restricted multipotential progenitor cells that in turn give rise to mature cells. The development of rigorous quantitative in vivo assays for HSC activity combined with multicolor flow cytometry and high-speed sorting have resulted in the phenotypic definition of HSCs to virtual purity. Here, we describe the isolation and identification of HSCs by flow cytometry and the use of competitive repopulation to assess HSC number and function.
造血干细胞（HSCs）能够自我更新，并产生血液和免疫系统的所有细胞。随着造血干细胞的分化，它们会逐渐丧失自我更新能力，并产生谱系受限的多能祖细胞，这些祖细胞进而产生成熟细胞。用于造血干细胞活性的严格定量体内检测方法的发展，结合多色流式细胞术和高速分选技术，已实现了造血干细胞表型的近乎纯界定。在此，我们描述通过流式细胞术分离和鉴定造血干细胞，以及利用竞争性再增殖来评估造血干细胞数量和功能的方法。
