Spielmann H, Balls M, Brand M, Döring B, Holzhütter H G, Kalweit S, Klecak G, Eplattenier H L, Liebsch M, Lovell W W, Maurer T, Moldenhauer F, Moore L, Pape W J, Pfanenbecker U, Potthast J, De Silva O, Steiling W, Willshaw A
ZEBET, Berlin, Germany.
Toxicol In Vitro. 1994 Aug;8(4):793-6. doi: 10.1016/0887-2333(94)90069-8.
In a joint validation project eight laboratories from the European Cosmetic Industry Association (COLIPA) as well as FRAME (England) and ZEBET (Germany) are trying to develop validated in vitro methods to be incorporated into new international guidelines for acute phototoxicity testing. The first stage of the study involved selection of the most promising in vitro phototoxicity tests for further validation. 20 chemicals with known phototoxic properties (12 phototoxins, four UV-absorbing non-phototoxins and four non-UV absorbing non-phototoxins) were tested under identical conditions of UV exposure conditions (sun simulator, UVA 5 J/cm(2)) in a standardized cytotoxicity assay with Balb/c 3T3 fibroblasts (endpoint: neutral red uptake, NRU). 19 of the 20 chemicals were correctly classified by the 3T3 NRU phototoxicity test, and therefore, this simple assay for phototoxicity seems very promising and should be validated further.
在一个联合验证项目中，来自欧洲化妆品工业协会（COLIPA）以及英国FRAME和德国ZEBET的八个实验室正在尝试开发经过验证的体外方法，以纳入急性光毒性测试的新国际指南。该研究的第一阶段涉及选择最有前景的体外光毒性测试进行进一步验证。在相同的紫外线暴露条件下（太阳模拟器，UVA 5 J/cm²），使用Balb/c 3T3成纤维细胞在标准化细胞毒性试验中对20种具有已知光毒性特性的化学物质（12种光毒素、4种紫外线吸收性非光毒素和4种非紫外线吸收性非光毒素）进行测试（终点：中性红摄取，NRU）。20种化学物质中的19种通过3T3 NRU光毒性测试被正确分类，因此，这种简单的光毒性检测方法似乎非常有前景，应该进一步验证。
