循环机械应变后骨髓间充质干细胞中骨相关基因的表达：对牵张成骨的意义。
Expression of bone-related genes in bone marrow MSCs after cyclic mechanical strain: implications for distraction osteogenesis.
机构信息
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
出版信息
Int J Oral Sci. 2009 Sep;1(3):143-50. doi: 10.4248/IJOS.09021.
AIM
Understanding the response of mesenchymal stem cells (MSCs) to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis.
METHODOLOGY
In this study, a single period of cyclic mechanical stretch (0.5 Hz, 2,000 microepsilon) was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase (ALP) activity was examined. The mRNA expression of six bone-related genes (Ets-1, bFGF, IGF-II, TGF-beta, Cbfa1 and ALP) was detected using real-time quantitative RT-PCR.
RESULTS
The results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up-regulate the expression of these genes. A significant increase in Ets-1 expression was detected immediately after mechanical stimulation, but Cbfa1 expression became elevated later. The temporal expression pattern of ALP coincided perfectly with Cbfa1.
CONCLUSION
The results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone-related genes may play different roles in the response of MSCs to mechanical stimulation.
目的
了解骨髓间充质干细胞（MSCs）对机械应变的反应及其随后的基因表达模式，将拓宽我们对牵张成骨力学生物学的认识。
方法
在这项研究中，对大鼠骨髓间充质干细胞进行了单次周期性机械拉伸（0.5Hz，2000μ ε）。检测细胞增殖和碱性磷酸酶（ALP）活性。使用实时定量 RT-PCR 检测六个与骨相关的基因（Ets-1、bFGF、IGF-II、TGF-β、Cbfa1 和 ALP）的 mRNA 表达。
结果
结果表明，机械应变可促进 MSCs 增殖，增加 ALP 活性，并上调这些基因的表达。机械刺激后即刻检测到 Ets-1 表达显著增加，但 Cbfa1 表达升高较晚。ALP 的时间表达模式与 Cbfa1 完全吻合。
结论
本研究结果提示，机械应变可能作为一种刺激物，诱导 MSCs 向成骨细胞分化，这些与骨相关的基因在 MSCs 对机械刺激的反应中可能发挥不同的作用。
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