Department of Chemical and Biological Engineering University of Colorado, Boulder, Colorado 80309, USA.
Langmuir. 2010 Aug 17;26(16):13364-7. doi: 10.1021/la1023357.
Single-molecule total internal reflectance fluorescence microscopy was used to observe the dynamic behavior of >4000 bovine serum albumin objects at the silicone oil-water interface. The surface residence time distribution indicated the presence of three populations at the interface. Each population had a characteristic fluorescence intensity and distinctive interfacial diffusion behavior. Larger fluorescence intensity correlated with longer residence times and slower diffusion. These combined observations of fluorescence intensity, surface residence time, and interfacial diffusion suggested that the three populations represent monomers, dimers, and trimers respectively.
采用单分子全内反射荧光显微镜观察了 >4000 个牛血清白蛋白分子在硅油-水界面的动态行为。表面停留时间分布表明界面上存在三个分子群体。每个群体都具有特征荧光强度和独特的界面扩散行为。较大的荧光强度与较长的停留时间和较慢的扩散相关。这些荧光强度、表面停留时间和界面扩散的综合观察表明，这三个分子群体分别代表单体、二聚体和三聚体。
