Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of Korea.
FEMS Microbiol Ecol. 2010 Oct;74(1):93-102. doi: 10.1111/j.1574-6941.2010.00947.x. Epub 2010 Aug 2.
The relative genetic diversity of microcystin-producing Microcystis in the water and sediment of the Daechung Reservoir, Korea, was investigated over an entire year, including the cyanobacterial bloom season. The cells of potentially toxic Microcystis strains containing mcyJ genotypes and cells containing the genus-specific cpcBA gene were quantified by a real-time PCR. The ratio of cells with mcyJ genotypes to the total Microcystis population in the water body was the highest (68.3%) in August when the cyanobacterial bloom reached its peak and the microcystin concentration in the water began to increase. A denaturing gradient gel electrophoresis profile analysis of the mcyJ genotypes performed to monitor any changes in the toxic Microcystis population showed the appearance of new genotypes and the disappearance of existing genotypes in the reservoir water collected during the summer months, when compared with the profile for the samples collected in spring and autumn. However, very little change was observed over the course of the year as regards the population diversity of the sediment samples.
对韩国大昌水库全年（包括蓝藻水华季节）的水体和底泥中的产微囊藻毒素微囊藻的相对遗传多样性进行了研究。采用实时 PCR 定量测定了含有 mcyJ 基因型的潜在有毒微囊藻菌株的细胞和含有属特异性 cpcBA 基因的细胞。当水华达到顶峰且水中微囊藻毒素浓度开始增加时，水体中含有 mcyJ 基因型的细胞与总微囊藻种群的比例最高（8 月为 68.3%）。对 mcyJ 基因型进行变性梯度凝胶电泳图谱分析以监测有毒微囊藻种群的任何变化，结果表明，与春季和秋季采集的样本相比，在夏季采集的水库水样中出现了新的基因型，并且存在的基因型消失了。然而，就沉积物样本的种群多样性而言，全年的变化很小。
