Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Bone Marrow Transplant. 2011 Jun;46(6):806-12. doi: 10.1038/bmt.2010.194. Epub 2010 Aug 9.
The best time of G-CSF administration for PBPC collection remains to be defined. We aimed to identify optimal G-CSF administration timing for efficient autologous stem cell collection. A total of 262 lymphoma or multiple myeloma patients, who underwent PBPC collection from January 2000 to March 2008, were included. PBPCs were mobilized with chemotherapy followed by lenograstim at 10 μg/kg/day. Patients received lenograstim at 2000 hours, about half a day before leukapheresis (PM group) before November 2004, and at 0600 hours, 3 h before apheresis (AM group) subsequently. In the AM group, the median number of total collected CD34+ cells/kg was greater over a shorter duration of apheresis, and the median number of collected CD34+ cells/kg at first leukapheresis was larger. Stem cell collection efficacy (ratio of total collected CD34+ cells/kg per number of leukapheresis procedures) was higher, and proportion of patients who yielded an optimum harvest was larger. The statistically significant between-group difference was observed only in patients with high-dose CY chemotherapy for stem cell mobilization in subgroup analysis. The present study showed that G-CSF injection 3 h before apheresis improved the efficacy of autologous stem cell collection.
粒细胞集落刺激因子（G-CSF）给药用于外周血造血干细胞（PBPC）采集的最佳时间仍有待确定。本研究旨在确定最佳的 G-CSF 给药时间，以实现高效的自体干细胞采集。
2000 年 1 月至 2008 年 3 月期间，共纳入 262 例淋巴瘤或多发性骨髓瘤患者进行 PBPC 采集。采用化疗联合粒细胞集落刺激因子（10μg/kg/d）进行 PBPC 动员。患者于 2000 时（PM 组），即白细胞单采前约半天，或 0600 时（AM 组），即白细胞单采前 3h 接受粒细胞集落刺激因子治疗。在 AM 组，在更短的白细胞单采时间内，总采集的 CD34+细胞/kg 中位数更多，首次白细胞单采的 CD34+细胞/kg 中位数更大。干细胞采集效率（每例白细胞单采次数的总采集 CD34+细胞/kg 比值）更高，产生最佳采集效果的患者比例更大。仅在亚组分析中，对于接受高剂量环磷酰胺（CY）化疗进行干细胞动员的患者，组间存在统计学显著差异。本研究表明，白细胞单采前 3h 给予 G-CSF 可提高自体干细胞采集的效果。
