John Ps, Paulose Cs, Sreekanth R
Department of Orthopedics, Medical College, Kottayam, Kerala, India.
Indian J Orthop. 2010 Jul;44(3):252-6. doi: 10.4103/0019-5413.65142.
Spinal cord trauma is a major health problem with associated physical, social, economic and psychological sequelae. Despite many advances in research and treatment modalities, the pathophysiology of spinal cord injury remains unclear, and morbidity and mortality among these patients remain high. This experimental study investigates the regenerative cell proliferation effects of bone marrow supplemented with neurotransmitters combinations in the regeneration of spinal cord injury
Ethical Committee Clearance was obtained for animal study. All animal care and procedures were in accordance with the CPCSEA and National Institute of Health guidelines. Thirty Wistar rats with monoplegia following surgical hemitransection of the spinal cord were used for the study. Half of them were randomly selected as the test group and the rest as the control group. Spinal cord injury model of Wistar rats in the test group were treated by infusing a combination of neurotransmitters and bone marrow at the site of injury using a special polythene tube and reservoir for 21 days. In the control group of rats with monoplegia, normal saline was infused at the site of injury for 21 days. The observations are recorded along with results.
The monoplegia in the test group of rats recovered significantly (P value < 0.01) with supplementation of the bone marrow cells and neurotransmitters combination. In the control group of rats, there was no recovery. The reward-seeking locomotor test and sensory recovery test confirmed recovery from spinal cord injury in the test group with significance.
The neurotransmitters and bone marrow combination was responsible for functional recovery in the test group of rats with experimental spinal cord injury We believe that the combination of neurotransmitters along with bone marrow may be a scope of future research in patients with spinal cord injury.
脊髓损伤是一个重大的健康问题，会引发身体、社会、经济和心理等一系列后遗症。尽管在研究和治疗方式上取得了许多进展，但脊髓损伤的病理生理学仍不清楚，这些患者的发病率和死亡率仍然很高。本实验研究探讨补充神经递质组合的骨髓在脊髓损伤再生中的细胞增殖再生作用。
获得动物研究的伦理委员会批准。所有动物护理和操作均符合CPCSEA和美国国立卫生研究院的指南。30只脊髓半横断术后出现偏瘫的Wistar大鼠用于该研究。其中一半被随机选为试验组，其余为对照组。试验组的Wistar大鼠脊髓损伤模型通过使用特殊的聚乙烯管和储液器在损伤部位注入神经递质和骨髓的组合，持续21天。在偏瘫大鼠对照组中，在损伤部位注入生理盐水，持续21天。记录观察结果及结果。
补充骨髓细胞和神经递质组合后，试验组大鼠的偏瘫明显恢复（P值<0.01）。对照组大鼠没有恢复。奖赏性运动试验和感觉恢复试验证实试验组大鼠从脊髓损伤中恢复，具有显著性。
神经递质和骨髓的组合导致了试验组实验性脊髓损伤大鼠的功能恢复。我们认为神经递质与骨髓的组合可能是未来脊髓损伤患者研究的一个方向。
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