Wu Sufan, Suzuki Yoshihisa, Ejiri Yoko, Noda Toru, Bai Hongliang, Kitada Masaaki, Kataoka Kazuya, Ohta Masayoshi, Chou Hirotomi, Ide Chizuka
Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan.
J Neurosci Res. 2003 May 1;72(3):343-51. doi: 10.1002/jnr.10587.
Transplantation of bone marrow stromal cells (MSCs) has been regarded as a potential approach for promoting nerve regeneration. In the present study, we investigated the influence of MSCs on spinal cord neurosphere cells in vitro and on the regeneration of injured spinal cord in vivo by grafting. MSCs from adult rats were cocultured with fetal spinal cord-derived neurosphere cells by either cell mixing or making monolayered-feeder cultures. In the mixed cell cultures, neuroshpere cells were stimulated to develop extensive processes. In the monolayered-feeder cultures, numerous processes from neurosphere cells appeared to be attracted to MSCs. In an in vivo experiment, grafted MSCs promoted the regeneration of injured spinal cord by enhancing tissue repair of the lesion, leaving apparently smaller cavities than in controls. Although the number of grafted MSCs gradually decreased, some treated animals showed remarkable functional recovery. These results suggest that MSCs might have profound effects on the differentiation of neurosphere cells and be able to promote regeneration of the spinal cord by means of grafting.
骨髓基质细胞（MSCs）移植已被视为促进神经再生的一种潜在方法。在本研究中，我们通过移植研究了MSCs对体外脊髓神经球细胞以及体内损伤脊髓再生的影响。将成年大鼠的MSCs与胎儿脊髓来源的神经球细胞通过细胞混合或制作单层饲养层培养物进行共培养。在混合细胞培养物中，神经球细胞被刺激形成广泛的突起。在单层饲养层培养物中，神经球细胞的许多突起似乎被吸引到MSCs。在体内实验中，移植的MSCs通过增强损伤组织的修复促进了损伤脊髓的再生，与对照组相比，留下的空洞明显更小。尽管移植的MSCs数量逐渐减少，但一些接受治疗的动物显示出显著的功能恢复。这些结果表明，MSCs可能对神经球细胞的分化有深远影响，并且能够通过移植促进脊髓再生。
