Valdman Alexander, Häggarth Lars, Cheng Liang, Lopez-Beltran Antonio, Montironi Rodolfo, Ekman Peter, Egevad Lars
Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.
Anal Quant Cytol Histol. 2009 Dec;31(6):367-74.
To evaluate the involvement of thioredoxin reductase (TxnR), thioredoxin (Trx) and peroxiredoxins (Prdx) in prostate cancer (PCa) and to assess the potential prognostic importance of these redox-regulated pathways.
Expression of the isoforms TxnR2, Trx1 and Prdx2 was studied by immunohistochemistry on tissue microarrays (TMAs). In a prognostic TMA, 294 primary cases of PCa with a median follow-up of 49 months were stained for Trx1 and Prdx2. Another TMA containing benign prostatic tissue, atrophy, high grade prostatic intraepithelial neoplasia (HGPIN) and PCa from 40 patients was stained with all 3 antibodies. RES ULTS: All 3 proteins showed similar expression patterns, with the highest immunoreactivity in HGPIN followed by atrophy, PCa and benign tissue. TxnR2, Trx1 and Prdx2 were overexpressed in HGPIN and PCa compared with benign tissue (p < 0.001), and Trx1 and Prdx2 were also overexpressed in HGPIN compared with PCa (p < 0.001). Trx1 and Prdx2 did not correlate with biochemical recurrence.
This study demonstrates up-regulation of the redox pathway proteins in PCa and its precursor lesions. The pathogenetic role of the redox system remains to be investigated.
评估硫氧还蛋白还原酶（TxnR）、硫氧还蛋白（Trx）和过氧化物酶（Prdx）在前列腺癌（PCa）中的作用，并评估这些氧化还原调节途径的潜在预后重要性。
通过免疫组织化学方法在组织微阵列（TMA）上研究TxnR2、Trx1和Prdx2亚型的表达。在一个预后TMA中，对294例原发性PCa病例（中位随访时间为49个月）进行Trx1和Prdx2染色。另一个包含40例患者的良性前列腺组织、萎缩、高级别前列腺上皮内瘤变（HGPIN）和PCa的TMA用所有3种抗体进行染色。结果：所有3种蛋白显示出相似的表达模式，在HGPIN中免疫反应性最高，其次是萎缩、PCa和良性组织。与良性组织相比，TxnR2、Trx1和Prdx2在HGPIN和PCa中过表达（p < 0.001），与PCa相比，Trx1和Prdx2在HGPIN中也过表达（p < 0.001）。Trx1和Prdx2与生化复发无关。
本研究表明PCa及其前驱病变中氧化还原途径蛋白上调。氧化还原系统的致病作用仍有待研究。
