Brönnegård M, Okret S
Department of Pediatrics, Huddinge University Hospital, Sweden.
J Steroid Biochem Mol Biol. 1991 Jul;39(1):13-7. doi: 10.1016/0960-0760(91)90006-q.
The effect of the synthetic glucocorticoid betamethasone on the regulation of the glucocorticoid receptor mRNA and on receptor protein was studied in fetal rat lung during development. Using a glucocorticoid receptor cRNA probe, glucocorticoid receptor mRNA was examined by Northern blot hybridization and by solution hybridization. A monoclonal antibody against the glucocorticoid receptor was used to study regulation of the receptor protein by the Western immunoblotting technique. In fetal rat lungs, of 16-21 days of gestation, as well as in adult lungs, betamethasone treatment resulted in a significant decrease of glucocorticoid receptor mRNA to 50-65% of the control level. In contrast, betamethasone treatment did not down-regulate the receptor protein in rat lungs of 16-19 days of gestation, whereas a decrease of glucocorticoid receptor protein to 40-60% of control was seen in lungs of 21 days of gestation, in postnatal and adult lung. These results provide data for a change in regulation in vivo of the glucocorticoid receptor by its homologous ligand in fetal rat lung during development.
在发育过程中，研究了合成糖皮质激素倍他米松对胎鼠肺中糖皮质激素受体mRNA调控及受体蛋白的影响。使用糖皮质激素受体cRNA探针，通过Northern印迹杂交和溶液杂交检测糖皮质激素受体mRNA。利用抗糖皮质激素受体单克隆抗体，采用Western免疫印迹技术研究受体蛋白的调控。在妊娠16 - 21天的胎鼠肺以及成年肺中，倍他米松处理导致糖皮质激素受体mRNA显著降低至对照水平的50 - 65%。相比之下，倍他米松处理在妊娠16 - 19天的大鼠肺中并未下调受体蛋白，而在妊娠21天的肺、出生后及成年肺中，糖皮质激素受体蛋白降低至对照的40 - 60%。这些结果为发育过程中胎鼠肺中糖皮质激素受体在体内受其同源配体调控的变化提供了数据。
