Bardi G, Johansson B, Pandis N, Heim S, Mandahl N, Andrén-Sandberg A, Hägerstrand I, Mitelman F
Department of Clinical Genetics, Lund University Hospital, Sweden.
Genes Chromosomes Cancer. 1991 Mar;3(2):149-52. doi: 10.1002/gcc.2870030211.
Cytogenetic analysis of short-term cultures from six adenocarcinomas of the colon revealed trisomy 7 as a recurrent clonal chromosomal abnormality. In three tumors, +7 was the sole change. In the fourth carcinoma, two aberrant clones with simple numerical aberrations were detected; one with +7 and one with +3. Tumors 5 and 6 both displayed two completely different abnormal clones; one had numerous numerical and structural abnormalities and thus was undoubtedly representative of the cancer parenchyma, and the other had only +7. The karyotypic differences between the coexisting clones in the latter two cases seem to argue against an evolutionary scenario in which the karyotypically more complex clones have evolved from the clones carrying trisomy 7 only. Furthermore, in tumor 6 the metaphases with trisomy 7 were found in colonies of fibroblast-like cells whereas those with a large number of abnormalities grew in colonies of epithelial-like cells. The combined results indicate that mitoses with trisomy 7 as the sole chromosomal change do not represent the neoplastic parenchyma of colorectal adenocarcinomas.
对6例结肠腺癌短期培养物进行的细胞遗传学分析显示，7号染色体三体是一种反复出现的克隆性染色体异常。在3个肿瘤中，+7是唯一的变化。在第4例癌中，检测到两个具有简单数目异常的异常克隆；一个为+7，另一个为+3。肿瘤5和肿瘤6均显示出两个完全不同的异常克隆；一个有许多数目和结构异常，因此无疑代表癌实质，另一个只有+7。后两例中共存克隆之间的核型差异似乎与一种进化模式相悖，在这种模式中，核型更复杂的克隆是从仅携带7号染色体三体的克隆进化而来的。此外，在肿瘤6中，发现7号染色体三体的中期细胞存在于成纤维细胞样细胞集落中，而具有大量异常的中期细胞则生长在上皮样细胞集落中。综合结果表明，以7号染色体三体作为唯一染色体变化的有丝分裂并不代表大肠腺癌的肿瘤实质。
