Raymond Christopher S, Zhu Lei, Vogt Thomas F, Shin Myung K
Merck & Co., Inc., Rahway, New Jersey, USA.
Methods Enzymol. 2010;477:415-27. doi: 10.1016/S0076-6879(10)77021-X.
Expression of small hairpin RNA (shRNA) in mammalian cells can trigger potent RNAi-mediated gene silencing. The dominant-acting RNAi can often result in phenotypes similar to that of a null allele. Moreover, the generation of shRNA knockdown mice and subsequent phenotypic analysis can be achieved in a condensed timeline compared to that of conventional gene-targeting knockout strategies. Here, we discuss methods for the in vivo analysis of gene function in adult mouse tissues following tetracycline-induced RNA knockdown in a single-copy inducible polymerase III promoter-driven shRNA system.
小发夹RNA（shRNA）在哺乳动物细胞中的表达可引发强大的RNA干扰（RNAi）介导的基因沉默。显性作用的RNAi通常会导致与无效等位基因相似的表型。此外，与传统的基因靶向敲除策略相比，shRNA敲低小鼠的产生及随后的表型分析可以在更短的时间内完成。在此，我们讨论了在单拷贝诱导型聚合酶III启动子驱动的shRNA系统中，通过四环素诱导的RNA敲低后，对成年小鼠组织中基因功能进行体内分析的方法。
