World Premier International Research (WPI) Center Initiative on Materials Nanoarchitronics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan.
Nanotechnology. 2010 Sep 10;21(36):365501. doi: 10.1088/0957-4484/21/36/365501. Epub 2010 Aug 11.
Vitamin C is an essential biological molecule for living organisms. The detection of vitamin C is always required due to its wide use in chemical, biological and pharmaceutical engineering. Here, we established a novel sensing system for rapid, ultrasensitive and highly selective detection of vitamin C based on a 'turn-on' fluorescent method. The turn-on fluorescent sensing system was built up of a colloidal CePO(4):Tb nanocrystalline solution with its fluorescence quenched by KMnO(4). The addition of vitamin C leads to a linear increase of fluorescence. The sensing principle of nanocrystalline CePO(4):Tb is based on a redox reaction via simply modulating the surface chemistry of nanocrystals. Our present sensing system for vitamin C exhibits a rapid response rate of less than 2 min, and highly selective and ultrasensitive detection with a detection limit of 108 nM, which is two orders of magnitude lower than that acquired by previously reported methods. The repeated reversibility of fluorescence quenching/recovery with time revealed a high reproducibility and long-term stability of our sensing materials. Furthermore, our developed sensing material overcomes the disadvantages such as complex surface modification/immobilization and serious biotoxicity compared to quantum-dot-based fluorescent sensing systems.
维生素 C 是生物体内必需的生物分子。由于其在化学、生物和制药工程中的广泛应用，维生素 C 的检测一直是必需的。在这里，我们建立了一种基于“开启”荧光法的新型快速、高灵敏、高选择性检测维生素 C 的传感系统。“开启”荧光传感系统由胶体 CePO(4):Tb 纳米晶溶液组成，其荧光被 KMnO(4)猝灭。加入维生素 C 会导致荧光线性增加。纳米晶 CePO(4):Tb 的传感原理基于通过简单调节纳米晶体的表面化学进行的氧化还原反应。我们目前用于检测维生素 C 的传感系统具有小于 2 分钟的快速响应速率，以及高度选择性和超灵敏检测，检测限为 108 nM，比以前报道的方法低两个数量级。荧光猝灭/恢复随时间的重复性显示出我们传感材料的高重现性和长期稳定性。此外，与基于量子点的荧光传感系统相比，我们开发的传感材料克服了复杂的表面修饰/固定化和严重的生物毒性等缺点。
