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利用嵌合聚羟基脂肪酸酯合酶在重组真养产碱杆菌和恶臭假单胞菌中生产聚（3-羟基丁酸酯-共-3-羟基己酸酯-共-3-羟基辛酸酯）三元共聚物

Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) terpolymers using a chimeric PHA synthase in recombinant Ralstonia eutropha and Pseudomonas putida.

作者信息

Sun Jian, Shozui Fumi, Yamada Miwa, Matsumoto Ken'ichiro, Takase Kazuma, Taguchi Seiichi

机构信息

Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-ku, Sapporo, Japan.

出版信息

Biosci Biotechnol Biochem. 2010;74(8):1716-8. doi: 10.1271/bbb.100242. Epub 2010 Aug 7.

DOI:10.1271/bbb.100242
PMID:20699558
Abstract

Recombinant strains of Ralstonia eutropha and Pseudomonas putida harboring a chimeric polyhydroxyalkanoate (PHA) synthase, which consisted of PHA synthases of Aeromonas caviae and R. eutropha, produced 3-hydroxybutyrate (3HB)-based PHA copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87-97 mol % 3HB), indicating that the chimeric PHA synthase possesses desirable substrate specificity leading to the production of 3HB-rich copolymers.

摘要

带有嵌合聚羟基脂肪酸酯（PHA）合酶的真养产碱菌和恶臭假单胞菌重组菌株产生了基于3-羟基丁酸（3HB）的PHA共聚物，该嵌合PHA合酶由豚鼠气单胞菌和真养产碱菌的PHA合酶组成，这些共聚物包含来自十二烷酸的3-羟基己酸酯和3-羟基辛酸酯单元（87-97摩尔%的3HB），这表明嵌合PHA合酶具有理想的底物特异性，能够产生富含3HB的共聚物。

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