利用假单胞菌属61-3聚羟基脂肪酸酯（PHA）合酶的酶同源物作为突变模板，探索该酶的一种新的有益突变，以提高基于3-羟基丁酸的PHA的细胞含量。
A new beneficial mutation in pseudomonas sp. 61-3 polyhydroxyalkanoate (PHA) synthase for enhanced cellular content of 3-hydroxybutyrate-based PHA explored using its enzyme homolog as a mutation template.
作者信息
Shozui Fumi, Sun Jian, Song Yuyang, Yamada Miwa, Sakai Kohei, Matsumoto Ken'ichiro, Takase Kazuma, Taguchi Seiichi
机构信息
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-ku, Sapporo, Japan.
出版信息
Biosci Biotechnol Biochem. 2010;74(8):1710-2. doi: 10.1271/bbb.100224. Epub 2010 Aug 7.
A newly isolated mutation (Gln508Leu) and a combination of it with previously discovered beneficial mutations in polyhydroxyalkanoate synthase 1 from Pseudomonas sp. 61-3 were found to enhance the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3HB-co-3-hydroxyalkanoate)s in recombinant Escherichia coli.
在重组大肠杆菌中发现了一种新分离的突变（Gln508Leu）及其与先前在假单胞菌属61-3的聚羟基脂肪酸酯合酶1中发现的有益突变的组合，可提高聚（3-羟基丁酸酯）[P（3HB）]和聚（3HB-共-3-羟基脂肪酸酯）的产量。
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