Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Bioprocess Biosyst Eng. 2011 Feb;34(2):173-7. doi: 10.1007/s00449-010-0458-y. Epub 2010 Aug 11.
Evaluation of metabolic pathways is one of the challenging areas in biological and pharmaceutical sciences. Phenanthridine oxidation to phenanthridinone is used commonly to study aldehyde oxidase activity. This reaction could pass through phenanthridine N-oxide intermediate. In the present study, the application of multivariate curve resolution, optimized by alternating least squares (MCR-ALS) to investigate this metabolic pathway has been described. The results obtained from MCR-ALS analysis along with those obtained from the use of potassium ferrocyanide method indicated that phenanthridine is directly oxidized to phenanthridinone by rat liver aldehyde oxidase without passing through phenanthridine N-oxide intermediate. It was also found that the later compound is not metabolized by this enzyme.
代谢途径的评估是生物和制药科学中的一个具有挑战性的领域。将菲啶氧化为菲啶酮通常用于研究醛氧化酶的活性。该反应可以经过菲啶 N-氧化物中间体。在本研究中，描述了通过交替最小二乘法（MCR-ALS）优化的多元曲线分辨（MCR-ALS）在研究这种代谢途径中的应用。MCR-ALS 分析得到的结果以及使用铁氰化钾方法得到的结果表明，大鼠肝醛氧化酶可直接将菲啶氧化为菲啶酮，而不经过菲啶 N-氧化物中间体。还发现该化合物不能被该酶代谢。
