Department of Orthopaedics, University of Pécs, Ifjúság u. 13, 7624, Pécs, Hungary.
Clin Orthop Relat Res. 2010 Nov;468(11):3112-20. doi: 10.1007/s11999-010-1443-0. Epub 2010 Aug 11.
Hyaline articular cartilage has limited repair and regeneration capacity. Intraarticular administration of glucocorticoid and local anesthetic injections play an important role in the therapy of osteoarthritis. Glucocorticoids and anesthetics reportedly enhance apoptosis in chondrocytes, but effects of the combined use of glucocorticoids and local anesthetics are unknown.
QUESTIONS/PURPOSES: We asked whether glucocorticoid and local anesthetic agents combined had any synergistic effects on chondrocyte apoptosis.
Cell viability and apoptosis/necrosis assessment of human articular chondrocytes were performed in vitro (chondrocyte cell cultures) and ex vivo (osteochondral specimens) using flow cytometry and TUNEL analysis, respectively.
Glucocorticoids and local anesthetics induce apoptosis in chondrocytes at various rates. When used in combination, the percentage of dead chondrocytes was increased in in vitro chondrocyte cell cultures and osteochondral ex vivo specimens.
We observed a time-dependent decrease in chondrocyte viability after concurrent steroid and local anesthetic exposure.
The combination of glucocorticoids and local anesthetics has an adverse effect on articular chondrocytes, and it raises a question regarding whether concomitant administration should be used in treating osteoarthritis.
透明软骨的修复和再生能力有限。关节内给予糖皮质激素和局部麻醉药注射在骨关节炎的治疗中起着重要作用。据报道，糖皮质激素和麻醉剂可增强软骨细胞的凋亡，但糖皮质激素和局部麻醉剂联合使用的效果尚不清楚。
问题/目的：我们想知道糖皮质激素和局部麻醉剂联合使用是否对软骨细胞凋亡有协同作用。
使用流式细胞术和 TUNEL 分析分别在体外（软骨细胞培养物）和体外（骨软骨标本）评估人关节软骨细胞的细胞活力和凋亡/坏死。
糖皮质激素和局部麻醉剂以不同的速度诱导软骨细胞凋亡。当联合使用时，体外软骨细胞培养物和骨软骨体外标本中死亡软骨细胞的百分比增加。
我们观察到在同时接触类固醇和局部麻醉剂后，软骨细胞的活力呈时间依赖性下降。
糖皮质激素和局部麻醉剂的联合使用对关节软骨有不良影响，这引发了一个问题，即在治疗骨关节炎时是否应同时使用。
