Toma Marieta I, Friedrich Katrin, Meyer Wolfdietrich, Fröhner Michael, Schneider Susanne, Wirth Manfred, Baretton Gustavo B
Institute for Pathology, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany.
Anal Quant Cytol Histol. 2010 Feb;32(1):1-10.
To analyze the centrosomal abnormalities in correlation with DNA ploidy and clinicopathologic data in prostate cancer.
Formalin-fixed, paraffin-embedded material from 63 prostate cancers (PCa) and 10 normal control cases were studied. Centrosomal features (number, area and shape) were assessed by immunohistochemistry with a gamma-tubulin monoclonal antibody. For each case centrosomal features were assessed in 100 cells, and the mean and median value was calculated. Statistical analysis was done by Student's t test, Mann-Whitney U test and multivariate analysis. The colocalization of gamma-tubulin and pericentrin at the centrosome was proven by double immunofluorescence staining. The DNA ploidy status was analyzed on Feulgen-stained, disintegrated paraffin sections using the OPTIMAS-based work station (Media Cybernetics, Silver Spring, Maryland, U.S.A.).
PCa cells showed centrosomal aberrations when compared to normal tissue. Poorly differentiated PCa showed more centrosomal abnormalities than well differentiated PCa (p < 0.05). Twenty-seven percent PCa were DNA nondiploid and 73% PCa were DNA diploid, respectively, just as all control specimens. DNA nondiploid status correlates with centrosomal abnormalities (p < 0.05). pT4 tumors showed significantly more centrosomes than pT2 and pT3 tumors (p < 0.05).
Changes in centrosome features indicate disturbed centrosome function and are significantly correlated with loss of differentiation in PCa. This is the first image analysis study of centrosome features in PCa, confirming that centrosome defects are involved in the acquisition of chromosomal aberrations in PCa.
分析前列腺癌中心体异常与DNA倍体及临床病理数据之间的相关性。
对63例前列腺癌（PCa）和10例正常对照病例的福尔马林固定、石蜡包埋材料进行研究。通过使用γ-微管蛋白单克隆抗体的免疫组织化学评估中心体特征（数量、面积和形状）。对每个病例在100个细胞中评估中心体特征，并计算平均值和中位数。采用学生t检验、曼-惠特尼U检验和多变量分析进行统计学分析。通过双重免疫荧光染色证实γ-微管蛋白和中心体蛋白在中心体处的共定位。使用基于OPTIMAS的工作站（美国马里兰州银泉市Media Cybernetics公司）在福尔根染色、脱蜡的石蜡切片上分析DNA倍体状态。
与正常组织相比，PCa细胞显示出中心体畸变。低分化PCa比高分化PCa表现出更多的中心体异常（p<0.05）。27%的PCa为DNA非整倍体，73%的PCa为DNA二倍体，所有对照标本情况相同。DNA非整倍体状态与中心体异常相关（p<0.05）。pT4肿瘤比pT2和pT3肿瘤显示出明显更多的中心体（p<0.05）。
中心体特征的变化表明中心体功能紊乱，并且与PCa的分化丧失显著相关。这是首次对PCa中心体特征进行的图像分析研究，证实中心体缺陷参与了PCa染色体畸变的发生。
