Sale G E, Farr A, Hamilton B L
Clinical Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Bone Marrow Transplant. 1991 Apr;7(4):263-7.
The minor histoincompatible mouse radiation chimera provides a useful model of graft-versus-host disease (GVHD) paralleling conditions in human marrow transplant recipients. While studying the B10.D2/BALB/c model, we noted that a graft-versus-host reaction of particular severity develops in the forestomach near the squamocolumnar junction. Comparison of the dyskeratotic index of this epithelium with that of the skin of the same animal revealed the forestomach to be a more sensitive site for detection of GVHD. In normal mice, both basal forestomach squamous epithelium and cells in the lamina propria expressed class II antigens. During the course of GVHD, class II antigen expression was elevated in the squamous epithelium of the forestomach, the columnar epithelium associated with the stomach, and cells within the associated lamina propria. The demonstration here that the squamocolumnar junction (considered to be a stem cell region for the maintenance of adjacent forestomach squamous epithelium) appears to be a particularly sensitive target tissue in GVHD and earlier identification of other epithelial target tissues of GVHD (hair follicles and rete ridges of epidermis, bile ductules of liver, and crypt cells of the gut) collectively support the hypothesis that GVHD tends to target sites of epithelial proliferation overlying epithelial stem cells.
小型组织相容性小鼠辐射嵌合体为人类骨髓移植受者中与移植物抗宿主病（GVHD）相似的情况提供了一个有用的模型。在研究B10.D2/BALB/c模型时，我们注意到在鳞状柱状交界处附近的前胃会发生特别严重的移植物抗宿主反应。将该上皮的角化不良指数与同一动物皮肤的角化不良指数进行比较，发现前胃是检测GVHD更敏感的部位。在正常小鼠中，前胃基底鳞状上皮和固有层中的细胞均表达II类抗原。在GVHD过程中，前胃鳞状上皮、与胃相关的柱状上皮以及相关固有层内的细胞中II类抗原表达升高。此处证明鳞状柱状交界处（被认为是维持相邻前胃鳞状上皮的干细胞区域）似乎是GVHD中特别敏感的靶组织，以及更早地识别GVHD的其他上皮靶组织（表皮的毛囊和 rete 嵴、肝脏的胆小管以及肠道的隐窝细胞）共同支持了GVHD倾向于靶向覆盖上皮干细胞的上皮增殖部位这一假说。
