Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Avda. República Saharaui, s/n, E-11510, Puerto Real, Cádiz, Spain.
J Fish Biol. 2010 Aug;77(3):719-30. doi: 10.1111/j.1095-8649.2010.02713.x.
The numbers of different types of ovarian follicles (developing, degenerating and postovulatory follicles) were estimated in bluefin tuna Thunnus thynnus using two stereological procedures: the model-based method of Weibel & Gomez, which has become a tool of broad application in the quantification of oocytes in fishes, and the assumption-free 'disector' (sic) method of Sterio. The estimates of developing follicles (follicles containing lipid-stage, vitellogenic and migratory-nucleus oocytes) made by the model-based method tended to be lower than those obtained with the disector, though significant differences were not observed except for vitellogenic follicles. Counts of atretic follicles by the model-based method were higher than those made using the disector, the differences being remarkable between both techniques, particularly in the case of beta-atresia, where the statistical analysis indicated significantly unequal estimations with the two methods. In contrast, the amount of postovulatory follicles estimated by the disector, which would stand for the realized batch fecundity, was somewhat larger than that calculated with the model-based method.
使用两种体视学程序估计了蓝鳍金枪鱼（Thunnus thynnus）的不同类型卵巢滤泡（发育滤泡、退化滤泡和排卵后滤泡）的数量：Weibel 和 Gomez 的基于模型的方法，该方法已成为鱼类卵母细胞定量的广泛应用工具，以及 Sterio 的无假设“切割器”（sic）方法。基于模型的方法估计的发育滤泡（含有脂质阶段、卵黄生成和迁移核卵母细胞的滤泡）数量往往低于切割器方法获得的数量，尽管除了卵黄生成滤泡外，没有观察到显著差异。基于模型的方法计数的退化滤泡数量高于切割器方法的数量，两种技术之间的差异非常显著，特别是在β退化的情况下，统计分析表明两种方法的估计存在显著差异。相比之下，切割器法估计的排卵后滤泡数量略大于基于模型的方法计算的数量，这代表了实际的批量繁殖力。
