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验证三种鱼类的血浆皮质醇和 11-酮睾酮酶免疫分析（EIA）试剂盒：橙小丑鱼 Amphiprion percula、橙鳍海葵鱼 Amphiprion chrysopterus 和黑鳍礁鲨 Carcharhinus melanopterus。

Plasma cortisol and 11-ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus.

机构信息

USR 3278 CNRS-EPHE, CRIOBE-CBETM, Université de Perpignan, 66860 Perpignan Cedex, France.

出版信息

J Fish Biol. 2010 Aug;77(3):769-77. doi: 10.1111/j.1095-8649.2010.02693.x.

DOI:10.1111/j.1095-8649.2010.02693.x
PMID:20701653
Abstract

Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 microl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions.

摘要

市售酶联免疫吸附测定（EIA）试剂盒已通过验证，可用于测量三种鱼类血浆中的类固醇激素浓度：橙小丑鱼 Amphiprion percula、橙鳍海葵鱼 Amphiprion chrysopterus 和黑鳍礁鲨 Carcharhinus melanopterus。两种试剂盒均需至少 5 微升血浆来估算激素浓度。这些 EIA 试剂盒是一种简单的方法，所需设备最少，可用于在野外条件下测量激素谱。

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