Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787, Japan.
J Struct Biol. 2011 Jan;173(1):67-76. doi: 10.1016/j.jsb.2010.08.004. Epub 2010 Aug 10.
Vibrio alginolyticus use flagella to swim. A flagellum consists of a filament, hook and basal body. The basal body is made up of a rod and several ring structures. This study investigates the structure of the T ring which is a unique component of the V. alginolyticus sodium ion-driven flagellar basal body. Using Zernike phase contrast (ZPC) cryo-electron tomography, we compared the 3D structures of purified hook-basal bodies (HBB) from a wild-type strain (KK148) and a deletion mutant lacking MotX and MotY (TH3), which are thought to form the T ring. ZPC images of HBBs had highly improved signal-to-noise ratio compared to conventional phase contrast images. We observed the outline of the HBBs from strains KK148 and TH3, and the TH3 mutant was missing its T ring. In the wild-type strain, the T ring was beneath the LP ring and seemed to form a ring shape with diameter of 32 nm.
噬藻弧菌利用鞭毛游动。鞭毛由丝状体、钩和基体组成。基体由一个杆和几个环结构组成。本研究调查了 T 环的结构，T 环是独特的一种弧菌钠离子驱动鞭毛基体的组成部分。使用泽尼克相衬（ZPC）低温电子断层扫描，我们比较了来自野生型菌株（KK148）和缺失 MotX 和 MotY（TH3）的缺失突变体的纯化钩-基体（HBB）的三维结构，这些蛋白被认为形成了 T 环。与传统的相衬图像相比，HBB 的 ZPC 图像具有更高的信噪比。我们观察到来自 KK148 和 TH3 菌株的 HBB 的轮廓，并且 TH3 突变体缺失了 T 环。在野生型菌株中，T 环位于 LP 环下方，似乎形成一个直径为 32nm 的环。
