Huang Ping, Tuo Ya, Wang Zhen-Yuan
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, P R China.
Fa Yi Xue Za Zhi. 2010 Jun;26(3):198-201.
Estimation of the postmortem interval (PMI) is a practical task in daily forensic casework. Researches on PMI is an important practical project in forensic field. Estimation of the time since death is influenced by internal and external, antemortem and postmortem factors, thus the old methods have limitations. Fourier transform infrared (FTIR) spectroscopy has been applied to study the pure protein, nucleic acid and carbohydrate and to detect the changes in complex cells and tissues. At present because the powerful software has could be used to achieve the spectrum transformation, smoothing, baseline correction and normalization, it is possible to analyze the samples quantitatively with the FTIR which has been applied in the biology and clinical medicine. This paper has reviewed the mechanism of FTIR and its application in biomedicine. The postmortem FTIR spectral changes were also discussed, which showed its potential for estimating PMI.
死后间隔时间（PMI）的估计是日常法医案件工作中的一项实际任务。PMI研究是法医领域的一个重要实际项目。死亡时间的估计受到内部和外部、生前和死后因素的影响，因此旧方法存在局限性。傅里叶变换红外（FTIR）光谱已被应用于研究纯蛋白质、核酸和碳水化合物，并检测复杂细胞和组织中的变化。目前，由于强大的软件可用于实现光谱变换、平滑、基线校正和归一化，因此使用FTIR对样品进行定量分析成为可能，FTIR已应用于生物学和临床医学。本文综述了FTIR的机制及其在生物医学中的应用。还讨论了死后FTIR光谱变化，显示了其在估计PMI方面的潜力。
