Li Wen-can, Zhang Ping, Chen Long
Department of Forensic Medicine, Shanghai Medical College of Fudan University, Shanghai 200032, China.
Fa Yi Xue Za Zhi. 2011 Feb;27(1):50-3.
Accurate estimation of postmortem interval (PMI) is one of the most important and difficult issues in forensic medicine. After death, the tissues autolyze and biomacromolecules degrade. DNA concentration decreases gradually with linear relationship with PMI. The housekeeping gene mRNA, for example beta-actin, GAPDH, has certain stability and can be used to PMI estimation as internal standard. This paper reviews the research progress and problems about DNA, RNA and proteins in the estimation of PMI in order to provide guidance for forensic pathology.
准确估计死后间隔时间（PMI）是法医学中最重要且最具挑战性的问题之一。死后，组织会自溶，生物大分子会降解。DNA浓度随PMI呈线性关系逐渐降低。管家基因mRNA，如β-肌动蛋白、甘油醛-3-磷酸脱氢酶，具有一定稳定性，可作为内标用于PMI估计。本文综述了DNA、RNA和蛋白质在PMI估计方面的研究进展与问题，旨在为法医病理学提供指导。
