School of Biological Sciences, Flinders University, G.P.O. Box 2100, Adelaide, SA 5001, Australia.
Comput Biol Chem. 2010 Aug;34(4):251-7. doi: 10.1016/j.compbiolchem.2010.07.001. Epub 2010 Jul 17.
An alternative treatment for staphylococcal infections caused by antibiotic-resistance strains is to lyse staphylococci with peptidoglycan hydrolases, for example, a cysteine, histidine-dependent amidohydrolase/peptidase (CHAP). Here, CHAPs were analyzed in 12 Staphylococcus aureus genomes and 44 staphylococcal phage genomes. There are 234 putative CHAP-containing proteins and only 64 non-identical CHAP sequences. These CHAPs can be classified into phage CHAPs encoded in phages/prophages and bacterial CHAPs encoded on chromosomes and plasmids. The phage CHAPs contain a sequence signature 'F-[IV]-R', and the bacterial CHAPs mainly do not. The phage CHAPs are mostly positioned at the protein N-termini whereas the bacterial CHAPs are all positioned at the C-termini. The cell wall targeting domains LysM and SH3_5 are associated with the bacterial CHAPs and the phage CHAPs, respectively. The homology modeling reveals that five of six highly conserved residues are clustered at the putative active site and are exposed to the molecular surface.
一种替代治疗抗生素耐药菌株引起的葡萄球菌感染的方法是用肽聚糖水解酶裂解葡萄球菌，例如半胱氨酸、组氨酸依赖性氨酰水解酶/肽酶 (CHAP)。在这里，在 12 个金黄色葡萄球菌基因组和 44 个葡萄球菌噬菌体基因组中分析了 CHAP。有 234 个假定含有 CHAP 的蛋白质，只有 64 个非相同的 CHAP 序列。这些 CHAP 可以分为噬菌体编码的 CHAP（在噬菌体/前噬菌体中编码）和细菌编码的 CHAP（在染色体和质粒上编码）。噬菌体 CHAP 含有序列特征 'F-[IV]-R'，而细菌 CHAP 主要没有。噬菌体 CHAP 大多位于蛋白质 N 末端，而细菌 CHAP 均位于 C 末端。细胞壁靶向结构域 LysM 和 SH3_5 分别与细菌 CHAP 和噬菌体 CHAP 相关联。同源建模显示，六个高度保守残基中的五个聚集在假定的活性部位并暴露在分子表面上。
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