Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore.
Biomaterials. 2010 Nov;31(33):8732-40. doi: 10.1016/j.biomaterials.2010.07.080. Epub 2010 Aug 14.
This work presents a modified method, namely coaxial electrohydrodynamic atomization, for the preparation of microspheres with distinct core/shell structures. This allows the encapsulation of two drugs with different characteristics in hydrophilic properties in one single step. Variation of ratios between outer flow and inner flow produces polymer microspheres with different core/shell ratios, and consequently results in variable release rates of drugs. Significant changes in release patterns were demonstrated when the distributions of the two drugs in microspheres were swapped. Moreover, cell culture experiments and animal experiments have been carried out to testify the performances of different microspheres in cytotoxicity, cellular apoptosis in vitro and tumor inhibition against subcutaneous U87 glioma xenograft in BALB/c nude mice. These findings present the advantages and possible application of this kind of multi-drug release system in treating brain tumors. Moreover, the release rates and characteristic sequences of multi-drugs can be tailored and tuned according to treatment necessity and applied in treating other kinds of tumors.
本工作提出了一种改进的方法，即同轴电动力学雾化法，用于制备具有明显核壳结构的微球。这使得能够在一步中将两种具有不同亲水性的药物封装在一起。通过改变外相和内相流速的比值，可以得到具有不同核壳比的聚合物微球，从而导致药物释放速率的变化。当微球中两种药物的分布被交换时，释放模式发生了显著变化。此外，还进行了细胞培养实验和动物实验，以验证不同微球在体外细胞毒性、细胞凋亡以及对 BALB/c 裸鼠皮下 U87 神经胶质瘤异种移植瘤的肿瘤抑制方面的性能。这些发现展示了这种多药物释放系统在治疗脑肿瘤方面的优势和可能的应用。此外，可以根据治疗需要调整和定制多药物的释放速率和特征序列，并应用于治疗其他类型的肿瘤。
