• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Sss1p 蛋白对于完成蛋白转位通道的激活是必需的。

Sss1p is required to complete protein translocon activation.

机构信息

Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom.

出版信息

J Biol Chem. 2010 Oct 15;285(42):32671-7. doi: 10.1074/jbc.M110.128256. Epub 2010 Aug 13.

DOI:10.1074/jbc.M110.128256
PMID:20709746
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2952269/
Abstract

Protein translocation across the endoplasmic reticulum membrane occurs at the Sec61 translocon. This has two essential subunits, the channel-forming multispanning membrane protein Sec61p/Sec61α and the tail-anchored Sss1p/Sec61γ, which has been proposed to "clamp" the channel. We have analyzed the function of Sss1p using a series of domain mutants and found that both the cytosolic and transmembrane clamp domains of Sss1p are essential for protein translocation. Our data reveal that the cytosolic domain is required for Sec61p interaction but that the transmembrane clamp domain is required to complete activation of the translocon after precursor targeting to Sec61p.

摘要

内质网膜上的蛋白易位发生在 Sec61 转运通道上。该通道由两个必需亚基组成，分别是形成通道的多跨膜蛋白 Sec61p/Sec61α和尾部锚定的 Sss1p/Sec61γ，后者被认为可以“夹住”通道。我们使用一系列结构域突变体分析了 Sss1p 的功能，发现 Sss1p 的胞质和跨膜夹区对于蛋白易位都是必需的。我们的数据表明，胞质结构域对于 Sec61p 的相互作用是必需的，但跨膜夹区对于前体靶向 Sec61p 后转运通道的完全激活是必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/753911629d34/zbc0451035390006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/7fc1d3f074ba/zbc0451035390001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/83cd7dea8527/zbc0451035390002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/82e5e3c577af/zbc0451035390003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/3c02412e83b9/zbc0451035390004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/cfc96bf25a05/zbc0451035390005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/753911629d34/zbc0451035390006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/7fc1d3f074ba/zbc0451035390001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/83cd7dea8527/zbc0451035390002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/82e5e3c577af/zbc0451035390003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/3c02412e83b9/zbc0451035390004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/cfc96bf25a05/zbc0451035390005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8972/2952269/753911629d34/zbc0451035390006.jpg

相似文献

1
Sss1p is required to complete protein translocon activation.Sss1p 蛋白对于完成蛋白转位通道的激活是必需的。
J Biol Chem. 2010 Oct 15;285(42):32671-7. doi: 10.1074/jbc.M110.128256. Epub 2010 Aug 13.
2
The conserved C-terminus of Sss1p is required to maintain the endoplasmic reticulum permeability barrier.Sss1p 的保守 C 端对于维持内质网通透性屏障是必需的。
J Biol Chem. 2020 Feb 14;295(7):2125-2134. doi: 10.1074/jbc.RA119.010378. Epub 2019 Dec 17.
3
Cancer associated mutations in Sec61γ alter the permeability of the ER translocase.Sec61γ 中的癌症相关突变改变了内质网易位酶的通透性。
PLoS Genet. 2021 Aug 30;17(8):e1009780. doi: 10.1371/journal.pgen.1009780. eCollection 2021 Aug.
4
The transmembrane domain is sufficient for Sbh1p function, its association with the Sec61 complex, and interaction with Rtn1p.跨膜结构域对于Sbh1p的功能、其与Sec61复合体的结合以及与Rtn1p的相互作用而言是足够的。
J Biol Chem. 2007 Oct 19;282(42):30618-28. doi: 10.1074/jbc.M701840200. Epub 2007 Aug 14.
5
Molecular architecture of the ER translocase probed by chemical crosslinking of Sss1p to complementary fragments of Sec61p.通过Sss1p与Sec61p互补片段的化学交联探究内质网转位酶的分子结构。
EMBO J. 1997 Aug 1;16(15):4549-59. doi: 10.1093/emboj/16.15.4549.
6
N-acetylation and phosphorylation of Sec complex subunits in the ER membrane.内质网膜中Sec复合体亚基的N-乙酰化和磷酸化。
BMC Cell Biol. 2012 Dec 13;13:34. doi: 10.1186/1471-2121-13-34.
7
SSS1 encodes a stabilizing component of the Sec61 subcomplex of the yeast protein translocation apparatus.SSS1编码酵母蛋白质转运装置的Sec61亚复合物的一个稳定成分。
J Biol Chem. 1994 Nov 4;269(44):27478-85.
8
Coordination of N-glycosylation and protein translocation across the endoplasmic reticulum membrane by Sss1 protein.Sss1蛋白在内质网膜上对N-糖基化和蛋白质转运的协调作用。
J Biol Chem. 2003 Sep 26;278(39):37998-8003. doi: 10.1074/jbc.M300176200. Epub 2003 Jul 14.
9
Stability and function of the Sec61 translocation complex depends on the Sss1p tail-anchor sequence.Sec61 易位复合物的稳定性和功能取决于 Sss1p 尾部锚定序列。
Biochem J. 2011 Jun 1;436(2):291-303. doi: 10.1042/BJ20101865.
10
Subunits of the translocon interact with components of the oligosaccharyl transferase complex.转位子的亚基与寡糖基转移酶复合体的组分相互作用。
J Biol Chem. 2005 Jun 17;280(24):22917-24. doi: 10.1074/jbc.M502858200. Epub 2005 Apr 14.

引用本文的文献

1
Cancer associated mutations in Sec61γ alter the permeability of the ER translocase.Sec61γ 中的癌症相关突变改变了内质网易位酶的通透性。
PLoS Genet. 2021 Aug 30;17(8):e1009780. doi: 10.1371/journal.pgen.1009780. eCollection 2021 Aug.
2
The conserved C-terminus of Sss1p is required to maintain the endoplasmic reticulum permeability barrier.Sss1p 的保守 C 端对于维持内质网通透性屏障是必需的。
J Biol Chem. 2020 Feb 14;295(7):2125-2134. doi: 10.1074/jbc.RA119.010378. Epub 2019 Dec 17.
3
N-terminal acetylation inhibits protein targeting to the endoplasmic reticulum.

本文引用的文献

1
Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome.单体酵母和哺乳动物质体 Sec61 复合物与翻译核糖体相互作用的结构。
Science. 2009 Dec 4;326(5958):1369-73. doi: 10.1126/science.1178535. Epub 2009 Oct 29.
2
The lateral gate of SecYEG opens during protein translocation.在蛋白质转运过程中，SecYEG的外侧门打开。
J Biol Chem. 2009 Jun 5;284(23):15805-14. doi: 10.1074/jbc.M901855200. Epub 2009 Apr 14.
3
Structure of a complex of the ATPase SecA and the protein-translocation channel.
N-端乙酰化抑制蛋白质靶向内质网。
PLoS Biol. 2011 May;9(5):e1001073. doi: 10.1371/journal.pbio.1001073. Epub 2011 May 31.
4
Stability and function of the Sec61 translocation complex depends on the Sss1p tail-anchor sequence.Sec61 易位复合物的稳定性和功能取决于 Sss1p 尾部锚定序列。
Biochem J. 2011 Jun 1;436(2):291-303. doi: 10.1042/BJ20101865.
ATP酶SecA与蛋白质转运通道复合物的结构
Nature. 2008 Oct 16;455(7215):936-43. doi: 10.1038/nature07335.
4
Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.Sec61和TRAP的单拷贝与非翻译状态的哺乳动物核糖体相关联。
Structure. 2008 Jul;16(7):1126-37. doi: 10.1016/j.str.2008.05.003.
5
Clustal W and Clustal X version 2.0.Clustal W和Clustal X 2.0版本
Bioinformatics. 2007 Nov 1;23(21):2947-8. doi: 10.1093/bioinformatics/btm404. Epub 2007 Sep 10.
6
Structure of the E. coli protein-conducting channel bound to a translating ribosome.与正在进行翻译的核糖体结合的大肠杆菌蛋白质传导通道的结构。
Nature. 2005 Nov 17;438(7066):318-24. doi: 10.1038/nature04133.
7
Protein translocation by the Sec61/SecY channel.通过Sec61/SecY通道进行的蛋白质转运
Annu Rev Cell Dev Biol. 2005;21:529-50. doi: 10.1146/annurev.cellbio.21.012704.133214.
8
X-ray structure of a protein-conducting channel.蛋白质传导通道的X射线结构
Nature. 2004 Jan 1;427(6969):36-44. doi: 10.1038/nature02218. Epub 2003 Dec 3.
9
Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum.翻译后转运蛋白进入内质网过程中Sec复合物与前体α因子之间的相互作用。
Mol Biol Cell. 2004 Jan;15(1):1-10. doi: 10.1091/mbc.e03-06-0390. Epub 2003 Nov 14.
10
Sec63p and Kar2p are required for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo.Sec63p和Kar2p是体内将SRP依赖性前体转运到酵母内质网中所必需的。
EMBO J. 2001 Jan 15;20(1-2):262-71. doi: 10.1093/emboj/20.1.262.