INRA, UMR408, Sécurité et Qualité des Produits d'Origine Végétale, Avignon, France.
Appl Environ Microbiol. 2010 Oct;76(19):6692-7. doi: 10.1128/AEM.00782-10. Epub 2010 Aug 13.
Bacillus cereus ATCC 14579 possesses five RNA helicase-encoding genes overexpressed under cold growth conditions. Out of the five corresponding mutants, only the ΔcshA, ΔcshB, and ΔcshC strains were cold sensitive. Growth of the ΔcshA strain was also reduced at 30°C but not at 37°C. The cold phenotype was restored with the cshA gene for the ΔcshA strain and partially for the ΔcshB strain but not for the ΔcshC strain, suggesting different functions at low temperature.
蜡样芽胞杆菌 ATCC 14579 在低温生长条件下过表达五个 RNA 解旋酶编码基因。在五个相应的突变体中,只有ΔcshA、ΔcshB 和ΔcshC 菌株对低温敏感。ΔcshA 菌株在 30°C 时的生长也受到抑制,但在 37°C 时不受影响。ΔcshA 菌株的 cshA 基因和部分ΔcshB 菌株的 cshA 基因可以恢复低温表型,但ΔcshC 菌株的 cshA 基因则不能,这表明它们在低温下具有不同的功能。