Department of Medicine, Hematology, University Hospital Basel, Basel, Switzerland.
Bone Marrow Transplant. 2011 May;46(5):650-8. doi: 10.1038/bmt.2010.193. Epub 2010 Aug 16.
The CD34(+) compartment of grafts for clinical allogeneic hematopoietic cell transplantation (HCT) is very heterogeneous. It contains hematopoietic stem cells and several different progenitor cell populations. This study assesses (1) the content of these populations in clinical grafts from G-CSF-mobilized PBMCs, BM and cord blood, (2) the functional correlation of the graft composition with time to engraftment of neutrophils, platelets and reticulocytes and (3) donor age-related changes. Quantitative flow cytometry showed that the distribution of the progenitor subsets differed significantly between the graft sources and that donor age-related changes occur. In patients after myeloablative allogeneic HCT, accelerated platelet and reticulocyte engraftment correlated with the content of common myeloid and/or megakaryocyte erythroid progenitors in the graft. These findings show that a better understanding of the progenitor compartment in human hematopoietic grafts could lead to improved strategies for the development of cellular therapies, for example in situations where platelet engraftment is delayed.
用于临床异基因造血细胞移植（HCT）的移植物的 CD34(+) 区室非常异质。它包含造血干细胞和几种不同的祖细胞群体。本研究评估了（1）从 G-CSF 动员的 PBMC、BM 和脐血的临床移植物中这些群体的含量，（2）移植物组成与中性粒细胞、血小板和网织红细胞植入时间的功能相关性，以及（3）供者年龄相关的变化。定量流式细胞术显示，祖细胞亚群在移植物来源之间的分布差异显著，并且存在供者年龄相关的变化。在清髓性异基因 HCT 后的患者中，血小板和网织红细胞的加速植入与移植物中共同髓系和/或巨核细胞红细胞祖细胞的含量相关。这些发现表明，更好地了解人类造血移植物中的祖细胞区室可能会导致细胞治疗策略的改进，例如在血小板植入延迟的情况下。
