Theilgaard-Mönch K, Raaschou-Jensen K, Schjødt K, Heilmann C, Vindeløv L, Jacobsen N, Dickmeiss E
Department of Hematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Bone Marrow Transplant. 2003 Dec;32(12):1125-33. doi: 10.1038/sj.bmt.1704297.
The present study compared the contents of pluripotent and lineage-committed hematopoietic progenitor cells (HPCs) in various types of allografts. Bone marrow (BM) allografts and single leukapheresis products (LPs) collected from G-CSF-mobilized donors contained similar amounts of pluripotent HPCs (CD34(+)CD38(-)) and total CD34(+) cells. However, the content of late-myeloid HPCs (CD34(+)CD33(+)CD15(+)) were significantly higher in BM grafts compared to LPs (P>0.02), whereas the contents of early-myeloid HPCs (CD34(+)CD33(+)CD15-) were 2.5-fold higher in LPs (P<0.03). In comparison to grafts from adult donors, cord blood (CB) grafts contained 26-65-fold lower amounts of early-myeloid HPCs (P<0.001), but only 8-12-fold lower contents of pluripotent HPCs (P<0.04). Additional findings demonstrated that among all tested parameters the numbers of early-myeloid HPCs were the most accurate measure of the total colony-forming cell (CFC) numbers in allografts. Hence, the earlier engraftment observed after transplantation of LPs compared to BM grafts might be explained by the higher content of early-myeloid HPCs/CFCs in LPs. Moreover, the slow engraftment following CB transplantation might not be affected essentially by the low number of myeloid HPCs, but rather by pluripotent HPCs. Finally, this study reports a new gating strategy for the enumeration of pluripotent CD34(+)CD38(-) subsets.
本研究比较了不同类型同种异体移植物中多能和定向造血祖细胞（HPCs）的含量。从粒细胞集落刺激因子（G-CSF）动员的供体采集的骨髓（BM）移植物和单次白细胞分离产物（LPs）含有相似数量的多能HPCs（CD34(+)CD38(-)）和总CD34(+)细胞。然而，与LPs相比，BM移植物中晚期髓系HPCs（CD34(+)CD33(+)CD15(+)）的含量显著更高（P>0.02），而早期髓系HPCs（CD34(+)CD33(+)CD15-）的含量在LPs中高2.5倍（P<0.03）。与成年供体的移植物相比，脐血（CB）移植物中早期髓系HPCs的含量低26 - 65倍（P<0.001），但多能HPCs的含量仅低8 - 12倍（P<0.04）。其他研究结果表明，在所有测试参数中，早期髓系HPCs的数量是同种异体移植物中总集落形成细胞（CFC）数量的最准确指标。因此，与BM移植物相比，LPs移植后观察到的更早植入可能是由于LPs中早期髓系HPCs/CFCs含量更高。此外，CB移植后植入缓慢可能主要不是受髓系HPCs数量少的影响，而是受多能HPCs的影响。最后，本研究报告了一种用于计数多能CD34(+)CD38(-)亚群的新的设门策略。
