Department of Clinical Neurobiology, Interdisciplinary Center for Neurosciences, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Exp Neurol. 2010 Nov;226(1):90-9. doi: 10.1016/j.expneurol.2010.08.006. Epub 2010 Aug 14.
In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in peri-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone.
在成年啮齿动物中，中风会促进神经发生，导致前脑区域（如纹状体或皮层）增加神经元，而在正常情况下，这些区域的神经发生作用可以忽略不计。在皮层中，新的神经元要么来自于受中风激活的局部皮层前体，要么来自于位于侧脑室室下区（SVZ）的前体，在正常情况下，SVZ 主要为嗅球提供神经母细胞。在这项研究中，我们使用了 5HT3A-EGFP 转基因小鼠，其中所有源自 SVZ 的神经母细胞均被 EGFP 标记。我们在这些小鼠中诱导中风，并结合 EGFP 检测和 BrdU 注射，标记所有中风后产生的 SVZ 源性神经母细胞。我们发现，中风后 14 天和 35 天，同侧半球的 SVZ 源性神经母细胞数量增加。中风后产生的 SVZ 源性神经母细胞迁移到皮层，并存活至少 35 天，占梗塞周围区域 BrdU 阳性细胞的 2%，在那里它们分化为成熟神经元。因此，中风增强了 SVZ 的神经发生，并吸引新生神经元到损伤区域。
