Department of Medical Physiology, Selcuk University, 42250, Konya, Turkey.
Department of Histology, Selcuk University, 42250, Konya, Turkey.
Neuromolecular Med. 2024 Mar 8;26(1):4. doi: 10.1007/s12017-023-08771-0.
Ischemic stroke is the leading cause of mortality and disability worldwide with more than half of survivors living with serious neurological sequelae; thus, it has recently attracted a lot of attention in the field of medical study.
The aim of this study was to determine the effect of naringin supplementation on neurogenesis and brain-derived neurotrophic factor (BDNF) levels in the brain in experimental brain ischemia-reperfusion.
The research was carried out on 40 male Wistar-type rats (10-12 weeks old) obtained from the Experimental Animals Research and Application Center of Selçuk University. Experimental groups were as follows: (1) Control group, (2) Sham group, (3) Brain ischemia-reperfusion group, (4) Brain ischemia-reperfusion + vehicle group (administered for 14 days), and (5) Brain ischemia-reperfusion + Naringin group (100 mg/kg/day administered for 14 days).
In the ischemia-reperfusion groups, global ischemia was performed in the brain by ligation of the right and left carotid arteries for 30 min. Naringin was administered to experimental animals by intragastric route for 14 days following reperfusion. The training phase of the rotarod test was started 4 days before ischemia-reperfusion, and the test phase together with neurological scoring was performed the day before and 1, 7, and 14 days after the operation. At the end of the experiment, animals were sacrificed, and then hippocampus and frontal cortex tissues were taken from the brain. Double cortin marker (DCX), neuronal nuclear antigen marker (NeuN), and BDNF were evaluated in hippocampus and frontal cortex tissues by Real-Time qPCR analysis and immunohistochemistry methods.
While ischemia-reperfusion increased the neurological score values, DCX, NeuN, and BDNF levels decreased significantly after ischemia in the hippocampus and frontal cortex tissues. However, naringin supplementation restored the deterioration to a certain extent.
The results of the study show that 2 weeks of naringin supplementation may have protective effects on impaired neurogenesis and BDNF levels after brain ischemia and reperfusion in rats.
缺血性中风是全球范围内导致死亡和残疾的主要原因，超过一半的幸存者留有严重的神经后遗症；因此，它最近在医学研究领域引起了广泛关注。
本研究旨在确定柚皮苷补充对实验性脑缺血再灌注后大脑神经发生和脑源性神经营养因子（BDNF）水平的影响。
这项研究是在 40 只 10-12 周龄雄性 Wistar 型大鼠（来自 Selçuk 大学实验动物研究与应用中心）身上进行的。实验组如下：（1）对照组，（2）假手术组，（3）脑缺血再灌注组，（4）脑缺血再灌注+载体组（给予 14 天），和（5）脑缺血再灌注+柚皮苷组（100mg/kg/天，给予 14 天）。
在缺血再灌注组中，通过结扎右侧和左侧颈总动脉 30 分钟来实现大脑的全局缺血。在再灌注后，通过灌胃途径向实验动物给予柚皮苷 14 天。在缺血再灌注之前的 4 天开始进行转棒测试的训练阶段，在手术前一天以及手术后第 1、7 和 14 天进行测试阶段和神经评分。在实验结束时，处死动物，然后从大脑中取出海马体和额叶皮质组织。通过实时 qPCR 分析和免疫组织化学方法评估海马体和额叶皮质组织中的双皮质标记物（DCX）、神经元核抗原标记物（NeuN）和 BDNF。
尽管缺血再灌注增加了神经评分值，但在海马体和额叶皮质组织中，缺血后 DCX、NeuN 和 BDNF 水平显著降低。然而，柚皮苷补充在一定程度上恢复了这种恶化。
研究结果表明，2 周的柚皮苷补充可能对大鼠脑缺血再灌注后受损的神经发生和 BDNF 水平具有保护作用。
