IEEE Trans Vis Comput Graph. 2011 Jun;17(6):857-70. doi: 10.1109/TVCG.2010.104. Epub 2010 Aug 5.
In this paper, we show that optical inverse tone-mapping (OITM) in light microscopy can improve the visibility of specimens, both when observed directly through the oculars and when imaged with a camera. In contrast to previous microscopy techniques, we premodulate the illumination based on the local modulation properties of the specimen itself. We explain how the modulation of uniform white light by a specimen can be estimated in real time, even though the specimen is continuously but not uniformly illuminated. This information is processed and back-projected constantly, allowing the illumination to be adjusted on the fly if the specimen is moved or the focus or magnification of the microscope is changed. The contrast of the specimen's optical image can be enhanced, and high-intensity highlights can be suppressed. A formal pilot study with users indicates that this optimizes the visibility of spatial structures when observed through the oculars. We also demonstrate that the signal-to-noise (S/N) ratio in digital images of the specimen is higher if captured under an optimized rather than a uniform illumination. In contrast to advanced scanning techniques that maximize the S/N ratio using multiple measurements, our approach is fast because it requires only two images. This can improve image analysis in digital microscopy applications with real-time capturing requirements.
在本文中，我们展示了光学逆色调映射（OITM）在光学显微镜中的应用，它可以提高标本的可视性，无论是通过目镜直接观察还是通过相机成像。与以前的显微镜技术不同，我们基于标本本身的局部调制特性来预先调制照明。我们解释了如何实时估计均匀白光通过标本的调制，即使标本是连续但不均匀照明的。这些信息会被不断处理和反向投影，因此如果标本移动或显微镜的焦点或放大倍数发生变化，照明可以实时调整。标本光学图像的对比度可以增强，高强度亮点可以被抑制。一项有用户参与的初步研究表明，当通过目镜观察时，这可以优化空间结构的可见度。我们还证明，如果在优化而非均匀照明下捕获标本的数字图像，其信号噪声比（S/N）更高。与使用多次测量来最大化 S/N 比的先进扫描技术不同，我们的方法速度很快，因为它只需要两张图像。这可以提高具有实时捕获要求的数字显微镜应用中的图像分析。
