The School of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, People's Republic of China.
Appl Microbiol Biotechnol. 2010 Oct;88(3):737-50. doi: 10.1007/s00253-010-2822-x. Epub 2010 Aug 17.
Jasmonates and nitric oxide (NO) play important roles in the regulation of the signaling network leading to the biosynthesis of plant secondary metabolites. In this work, we explore the effect of constitutive overexpression of CrORCA3 (octadecanoid-responsive Catharanthus AP2/ERF domain), methyl jasmonate (MeJA), and sodium nitroprusside (SNP) on the differentiated tissue of Catharanthus roseus hairy roots. The changes in catharanthine concentration and in the levels of mRNA transcripts of pathway genes and regulators were tracked for 192 h. ORCA3 overexpression led to a slight decrease of the accumulation of catharanthine, while MeJA treatment caused a large increase in the levels of transcripts of pathway genes and the catharanthine concentration. SNP treatment alone or SNP in combination with MeJA treatment caused a dramatic decrease of the cathanranthine concentration, while at the same time the levels of transcripts of zinc finger-binding proteins genes (ZCTs) increased. The latter treatment also caused a decrease of the levels of transcripts of type-I protein prenyltransferase gene (PGGT-I). This response of transcriptional repressors and pathway genes may explain the antagonistic effects of NO and MeJA on catharanthine biosynthesis in C. roseus hairy roots.
茉莉酸（Jasmonates）和一氧化氮（NO）在调控植物次生代谢物生物合成的信号转导网络中发挥着重要作用。在这项工作中，我们探讨了组成型过表达 CrORCA3（十八碳烯响应的长春花 AP2/ERF 结构域）、茉莉酸甲酯（MeJA）和硝普钠（SNP）对长春花毛状根分化组织的影响。跟踪了 192 小时内 Catharanthine 浓度的变化以及途径基因和调控因子的 mRNA 转录本水平。ORCA3 过表达导致 Catharanthine 的积累略有减少，而 MeJA 处理导致途径基因和 Catharanthine 浓度的转录本水平大幅增加。单独用 SNP 处理或 SNP 与 MeJA 联合处理导致 Catharanthine 浓度急剧下降，同时锌指结合蛋白基因（ZCTs）的转录本水平增加。后一种处理还导致 I 型蛋白 prenyltransferase 基因（PGGT-I）的转录本水平降低。转录抑制因子和途径基因的这种反应可能解释了 NO 和 MeJA 对长春花毛状根中 Catharanthine 生物合成的拮抗作用。
