Department of Microbiology, University of Barcelona, Diagonal 645, 08028 Barcelona, Spain.
Appl Microbiol Biotechnol. 2010 Oct;88(4):985-95. doi: 10.1007/s00253-010-2816-8. Epub 2010 Aug 17.
A chemical and microbial characterization of lab-scale biostimulation assays with groundwater samples taken from an industrial site in which the aquifer had been contaminated by linear non-sulfonate alkyl benzenes (LABs) was carried out for further field-scale bioremediation purposes. Two lab-scale biodegradability assays were performed, one with a previously obtained gas-oil-degrading consortium and another with the native groundwater flora. Results for the characterization of the groundwater microbial population of the site revealed the presence of an important LAB-degrading microbial population with a strong degrading capacity. Among the microorganisms identified at the site, the detection of Parvibaculum lavamentivorans, which have been described in other studies as alkyl benzene sulfonates degraders, is worth mentioning. Incubation of P. lavamentivorans DSMZ13023 with LABs as reported in this study shows for the first time the metabolic capacity of this strain to degrade such compounds. Results from the biodegradation assays in this study showed that the indigenous microbial population had a higher degrading capacity than the gas-oil-degrading consortium, indicating the strong ability of the native community to adapt to the presence of LABs. The addition of inorganic nutrients significantly improved the aerobic biodegradation rate, achieving levels of biodegradation close to 90%. The results of this study show the potential effectiveness of oxygen and nutrients as in situ biostimulation agents as well as the existence of a complex microbial community that encompasses well-known hydrocarbon- and LAS-degrading microbial populations in the aquifer studied.
对取自受直链非磺酸盐烷基苯 (LAB) 污染的工业场地含水层的地下水样本进行实验室规模生物刺激试验的化学和微生物特性分析，目的是进一步开展现场规模的生物修复。进行了两个实验室规模的生物降解性试验，一个使用先前获得的油气降解菌混合体，另一个使用原生地下水菌群。对该场地地下水微生物种群的特性进行的分析结果表明，存在着具有较强降解能力的重要 LAB 降解微生物种群。在该场地鉴定出的微生物中，值得一提的是发现了 Parvibaculum lavamentivorans，其他研究表明该菌可以降解烷基苯磺酸盐。本研究中报道的 P. lavamentivorans DSMZ13023 与 LAB 的共培养首次表明了该菌株降解此类化合物的代谢能力。本研究中生物降解性试验的结果表明，土著微生物种群的降解能力高于油气降解菌混合体，表明土著群落具有很强的适应 LAB 存在的能力。无机营养物的添加显著提高了好氧生物降解速率，达到接近 90%的生物降解水平。本研究结果表明，氧气和营养物作为原位生物刺激剂具有潜在的有效性，以及在研究的含水层中存在着包含已知烃类和 LAS 降解微生物种群的复杂微生物群落。
