Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-333, Republic of Korea.
Biotechnol Lett. 2010 Dec;32(12):1829-35. doi: 10.1007/s10529-010-0381-y. Epub 2010 Aug 17.
A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 μmol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P.
开发了一种混合厌氧微生物富集培养物 AMEC-4P，它使用乳酸盐作为电子供体，将四氯乙烯 (PCE) 还原脱氯为乙烯。AMEC-4P 可在 13 天内将 2 mM PCE 持续且完全地转化为顺式-1,2-二氯乙烯 (cis-DCE)，然后在 130 天后，中间产物 cis-DCE 完全脱氯为乙烯。PCE 到 cis-DCE、cis-DCE 到 VC 和 VC 到乙烯的脱氯速率分别为 243、27 和 41 μmol/l/天。根据 16S rRNA 序列鉴定出 Geobacter lovleyi 和 Dehalococcoides sp. 是 AMEC-4P 中的优势菌群。
