由沉积物中厌氧微生物富集培养物实现四氯乙烯的完全还原脱氯生成乙烯。
Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment.
机构信息
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-333, Republic of Korea.
出版信息
Biotechnol Lett. 2010 Dec;32(12):1829-35. doi: 10.1007/s10529-010-0381-y. Epub 2010 Aug 17.
A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 μmol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P.
开发了一种混合厌氧微生物富集培养物 AMEC-4P，它使用乳酸盐作为电子供体，将四氯乙烯 (PCE) 还原脱氯为乙烯。AMEC-4P 可在 13 天内将 2 mM PCE 持续且完全地转化为顺式-1,2-二氯乙烯 (cis-DCE)，然后在 130 天后，中间产物 cis-DCE 完全脱氯为乙烯。PCE 到 cis-DCE、cis-DCE 到 VC 和 VC 到乙烯的脱氯速率分别为 243、27 和 41 μmol/l/天。根据 16S rRNA 序列鉴定出 Geobacter lovleyi 和 Dehalococcoides sp. 是 AMEC-4P 中的优势菌群。
Zotero 插件